当前位置: X-MOL 学术Nat. Microbiol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A pathway for biological methane production using bacterial iron-only nitrogenase.
Nature Microbiology ( IF 28.3 ) Pub Date : 2018-Mar-01 , DOI: 10.1038/s41564-017-0091-5
Yanning Zheng , Derek F. Harris , Zheng Yu , Yanfen Fu , Saroj Poudel , Rhesa N. Ledbetter , Kathryn R. Fixen , Zhi-Yong Yang , Eric S. Boyd , Mary E. Lidstrom , Lance C. Seefeldt , Caroline S. Harwood

Methane (CH4) is a potent greenhouse gas that is released from fossil fuels and is also produced by microbial activity, with at least one billion tonnes of CH4 being formed and consumed by microorganisms in a single year 1 . Complex methanogenesis pathways used by archaea are the main route for bioconversion of carbon dioxide (CO2) to CH4 in nature2-4. Here, we report that wild-type iron-iron (Fe-only) nitrogenase from the bacterium Rhodopseudomonas palustris reduces CO2 simultaneously with nitrogen gas (N2) and protons to yield CH4, ammonia (NH3) and hydrogen gas (H2) in a single enzymatic step. The amount of CH4 produced by purified Fe-only nitrogenase was low compared to its other products, but CH4 production by this enzyme in R. palustris was sufficient to support the growth of an obligate CH4-utilizing Methylomonas strain when the two microorganisms were grown in co-culture, with oxygen (O2) added at intervals. Other nitrogen-fixing bacteria that we tested also formed CH4 when expressing Fe-only nitrogenase, suggesting that this is a general property of this enzyme. The genomes of 9% of diverse nitrogen-fixing microorganisms from a range of environments encode Fe-only nitrogenase. Our data suggest that active Fe-only nitrogenase, present in diverse microorganisms, contributes CH4 that could shape microbial community interactions.

中文翻译:

使用仅细菌铁的固氮酶生产生物甲烷的途径。

甲烷(CH 4)是一种强大的温室气体,从化石燃料中释放出来,也是通过微生物活动产生的,在一年中,至少有十亿吨的CH 4被微生物形成和消耗1。在古自然界2-4中,古细菌使用的复杂甲烷生成途径是二氧化碳(CO 2)到CH 4的生物转化的主要途径。在这里,我们报道来自细菌红假单胞菌的野生型铁(仅铁)固氮酶与氮气(N 2)和质子同时还原CO 2,生成CH 4,氨(NH 3)和氢气(H 2个)在一个酶促步骤中进行。CH的量4由纯化仅有Fe的固氮制得低相比,它的其它产品,但CH 4的生产通过在沼泽红假单胞菌这种酶足以支持一种专CH生长4 -utilizing甲基单应变时的两种微生物在共培养中生长,每隔一段时间添加氧气(O 2)。我们测试的其他固氮细菌也形成了CH 4当表达仅铁的固氮酶时,表明这是该酶的一般性质。来自各种环境的9%的各种固氮微生物的基因组均编码仅铁的固氮酶。我们的数据表明,存在于多种微生物中的仅活性Fe固氮酶有助于CH 4形成,可能影响微生物群落之间的相互作用。
更新日期:2018-01-15
down
wechat
bug