The front cover picture shows symbolically a high‐throughput silicon‐chip‐based DNA solid‐phase synthetic platform (background) for preparing a library of DNA mutants that encode epoxide hydrolase (center) variants. Massive sequencing revealed that 97 % of the theoretically possible DNA mutants were formed, compared to only 56 % when applying conventional saturation mutagenesis. The DNA synthetic approach is also superior at the protein level, with many stereoselective variants that were missed in the saturation mutagenesis library being identified. The picture was prepared by Gillian Jarvis and Aitao Li. More information can be found in the full paper by M. T. Reetz et al. on page 221 in Issue 3, 2018 (DOI: 10.1002/cbic.201700540).

中文翻译：

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封面：克服蛋白质定向进化的偏见：将高保真芯片固相基因合成与高效基因组装相结合，以进行组合文库构建（ChemBioChem 3/2018）

封面图片象征性地显示了一个高通量的基于硅芯片的DNA固相合成平台（背景），用于制备编码环氧化物水解酶（中心）变体的DNA突变体库。大规模测序表明，形成了97％的理论上可能的DNA突变体，而使用常规饱和诱变时只有56％。DNA合成方法在蛋白质水平上也很出色，可以鉴定出饱和诱变文库中遗漏的许多立体选择性变体。图片由吉莉安·贾维斯（Gillian Jarvis）和李爱陶（Aitao Li）制作。可以在M. T. Reetz等人的全文中找到更多信息。就在第3期，2018第221页（：10.1002 / cbic.201700540 DOI）。