The present study examined the potential of ethyl acetate fraction (EAF) isolated from Molineria latifolia rhizome to modulate the oxidant-antioxidant balance in high fat diet and nicotinamide/streptozotocin-induced diabetic rats. Administration with EAF ameliorated systemic antioxidant status- and oxidative stress-related parameters without affecting the renal and liver functions in the diabetic rats. Further analyses on adipose, muscle and liver tissues demonstrated differing ability to scavenge free radicals and protection against lipid peroxidation. Transcriptional changes proposed concerted modulation of both KEAP1-NRF2 and PKC/NF-κB signallings in tissue-specific manner. Qualitative profiling of compounds present in EAF was analysed by non-targeted HPLC-QTOF mass spectrometry. A total of 23 unique mass signals were detected in EAF. Putative identification revealed a mixture of naturally occurring polyphenols ranging from cinnamic acid-, benzoic acid- and flavonoid-derived groups. Overall, the study demonstrated potential application of EAF to reinstate diabetes-induced oxidant-antioxidant imbalance by potentially modulating the NRF2–NF-κB signalling axis.

本研究检查了从Mo木中分离出的乙酸乙酯级分（EAF）的潜力根茎调节高脂饮食和烟酰胺/链脲佐菌素诱导的糖尿病大鼠中的氧化剂-抗氧化剂平衡。用EAF给药可改善系统性抗氧化剂状态和氧化应激相关参数，而不会影响糖尿病大鼠的肾和肝功能。对脂肪，肌肉和肝组织的进一步分析表明，清除自由基和抵抗脂质过氧化的能力不同。转录变化建议以组织特异性方式协同调节KEAP1-NRF2和PKC /NF-κB信号。通过非目标HPLC-QTOF质谱分析了EAF中存在的化合物的定性分析。在EAF中总共检测到23个独特的质量信号。推定的鉴定显示，天然存在的多酚混合物包括肉桂酸，苯甲酸和类黄酮衍生的基团。总体而言，该研究表明，EAF可通过潜在地调节NRF2-NF-κB信号轴来恢复糖尿病引起的氧化剂-抗氧化剂失衡。