A metabolomics method based on ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) was used to investigate the effect of Lactobacillus plantarum NCU116 on the metabolites of hyperlipidaemic rat model feed by high-fat diet. Nine potential biomarkers (Pantothenic Acid, 5-Hydroxyindole acetaldehyde, Glycocholic Acid, Biliverdin IX, l-Leucine, 2-Phenylethanol glucuronide, Taurocholic acid, Indoleacrylic acid and 2-Arachidonoylglycerol) were identified and the pathway features were analyzed by Mass Profiler Professional (MPP) software. Meanwhile, clustering of principal components analysis (PCA), partial least squares-discriminant analysis (PLS-DA) and hierarchical clustering analysis (HCA) produces and biological pathways including tryptophan metabolism, bile secretion, fatty acid biosynthesis, glucose homeostasis, protein digestion and absorption were affected by L. plantarum NCU116. This study demonstrated that L. plantarum NCU116 improved hyperlipidaemic rats via metabolism pathways of amino acids, bile, fatty acids and glucose.

采用基于超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF / MS）的代谢组学方法研究植物乳杆菌NCU116对高脂饮食高脂血症大鼠模型饲料代谢产物的影响。九种潜在的生物标志物（泛酸，5-羟基吲哚乙醛，乙醇酸，Biliverdin IX，l鉴定了亮氨酸，2-苯基乙醇葡糖醛酸苷，牛磺胆酸，吲哚丙烯酸和2-花生四烯酸甘油），并通过Mass Profiler Professional（MPP）软件分析了路径特征。同时，主成分分析（PCA），偏最小二乘判别分析（PLS-DA）和分层聚类分析（HCA）的聚类产生了生物途径，包括色氨酸代谢，胆汁分泌，脂肪酸生物合成，葡萄糖稳态，蛋白质消化和吸收受到植物乳杆菌NCU116的影响。这项研究表明，植物乳杆菌NCU116通过氨基酸，胆汁，脂肪酸和葡萄糖的代谢途径改善了高脂血症大鼠。