Spent hens have low market value and incur significant costs for disposal. This study explored the use of spent hens as a source of bioactive peptides. Spent hen hydrolysates prepared by Protease M or Protex 50FP exhibited interleukin (IL)-6 inhibitory activity in endotoxin-activated macrophage-like U937 cells (p < .05). The potential peptides from Protex 50FP hydrolysate were further fractionated using a combination of ultrafiltration, solid-phase extraction and high-performance liquid chromatography; 17 novel peptides encoded in major muscle proteins were identified by mass spectrometry analysis, of which 7 were chemically synthesized and assayed for the IL-6 inhibitory activity. At a concentration of 100 µg/mL, peptide FLWGKSY induced a 79% reduction of IL-6 production in endotoxin-activated macrophage-like U937 cells, which is comparable to results reported from other food sources. Our results indicate that spent hens have potential to be a source of bioactive peptides for anti-inflammatory applications.

废母鸡的市场价值低，处置费用高。这项研究探索了将废母鸡用作生物活性肽的来源。由蛋白酶M或Protex 50FP制备的废母鸡水解产物在内毒素激活的巨噬细胞样U937细胞中表现出白介素（IL）-6抑制活性（p <.05）。通过结合使用超滤，固相萃取和高效液相色谱法进一步分离来自Protex 50FP水解产物的潜在肽。通过质谱分析鉴定出了在主要肌肉蛋白中编码的17种新肽，其中7种是化学合成并测定了IL-6抑制活性。在浓度为100 µg / mL的条件下，肽FLWGKSY可以诱导内毒素激活的巨噬细胞样U937细胞中IL-6的生成减少79％，这与其他食品来源的报告结果相当。我们的结果表明，废母鸡有潜力成为抗炎应用的生物活性肽的来源。