This study developed, optimized and validated an ultra-high-performance liquid chromatography–high-resolution mass spectrometry (UHPLC-HRMS) method to identify and quantify metabolites and microbial-derived catabolites in urine, plasma and feces of rats following ingestion of 50 mg of a red wine proanthocyanidin-rich extract. The method was validated for specificity, linearity, limit of detection (LD) and quantification (LQ), intra-day and inter-day precision, recovery and matrix effects, which were determined for 34 compounds in the three biological matrices. After method validation, three parent flavan-3-ols, four 5-carbon side chain ring fission metabolites, and 27 phenolic acid and aromatic catabolites were quantified in plasma, urine and feces after red wine proanthocyanidin intake. These results establish the value of the UHPLC-HRMS protocol in obtaining a detailed picture of proanthocyanidin metabolites and their microbial-derived catabolites, along with their phase II metabolites, in biological fluids of rat, and potentially in human clinical studies designed to evaluate the bioavailability of dietary flavan-3-ols.

这项研究开发，优化和验证了一种超高效液相色谱-高分辨率质谱（UHPLC-HRMS）方法，用于鉴定和定量摄入50 mg大鼠尿液，血浆和粪便中的代谢物和微生物衍生的分解代谢物红葡萄酒富含原花青素的提取物。验证了该方法的特异性，线性，检测限（LD）和定量（LQ），日内和日间精度，回收率和基质效应，对三种生物基质中的34种化合物进行了测定。在方法验证后，定量了红酒原花青素摄入后血浆，尿液和粪便中的三种母体黄烷-3-醇，四种5-碳侧链裂变代谢产物以及27种酚酸和芳香族分解代谢物的含量。