A high-performance size-exclusion chromatography (HPSEC) method coupled to Evaporative Light Scattering (ELS) and Refractive Index (RI) detectors were evaluated and compared for the molecular mass (Mw) estimation of pectin in a wide range (0.342–805 kDa). Instrumental parameters of the ELSD were optimised by Response Surface Methodology (RSM) being 73 °C the evaporator temperature and 0.9 mL/min the air flow rate. The linear range for the ELSD concentration response was wider (10–2250 mg/L) and better (R² = 0.985) than RID (10–1500 mg/L; R² = 0.875). The limits of detection (LOD) and quantitation (LOQ) for all pullulans hardly changed in ELSD (LOD: 1.22–1.99 mg/L; LOQ: 4.07–6.63 mg/L); however, RID showed huge variations (LOD: 0.49–10.41 mg/L; LOQ: 1.64–34.70 mg/L), which increased with the Mw. In general, responses of both detectors were similar for the Mw estimation, although pectin characterisation with HPSEC-ELSD exhibited better results in the lowest Mw compounds.

结合蒸发光散射（ELS）和折光指数（RI）检测器的高效尺寸排阻色谱（HPSEC）方法进行了评估，并比较了在宽范围（0.342–805 kDa）中果胶的分子量（Mw）估算值）。ELSD的仪器参数通过响应表面方法（RSM）进行了优化，该方法是蒸发器温度为73°C，空气流速为0.9 mL / min。ELSD浓度响应的线性范围 比RID（10–1500 mg / L; R ²）宽（10–2250 mg / L）和更好（R ² = 0.985）。 = 0.875）。ELSD中所有支链淀粉的检出限（LOD）和定量限（LOQ）几乎不变（LOD：1.22-1.99 mg / L； LOQ：4.07-6.63 mg / L）；然而，RID表现出巨大的变化（LOD：0.49–10.41 mg / L； LOQ：1.64–34.70 mg / L），并且随着Mw的增加而增加。通常，尽管用HPSEC-ELSD进行果胶表征在Mw最低的化合物中表现出更好的结果，但两种检测器的Mw估计值的响应是相似的。