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Secondary structure changes induced by pulsed electric field affect antioxidant activity of pentapeptides from pine nut (Pinus koraiensis) protein
Food Chemistry ( IF 8.8 ) Pub Date : 2018-01-12 , DOI: 10.1016/j.foodchem.2018.01.090
Rong Liang , Sheng Cheng , Xiuying Wang

We used a pulsed electric field (PEF) to treat four pentapeptides with similar amino acid sequences (KCHKP, KCHQP, QCHKP, and QCHQP). We then evaluated antioxidant activity of the pentapeptides according to ferric reducing antioxidant power (FRAP) and hydroxyl radical scavenging capacity. Structures of the peptides were determined by high performance liquid chromatography (HPLC), mid-infrared (MIR), circular dichroism (CD) spectroscopy, and nuclear magnetic resonance (NMR). The results indicated that antioxidant activities of KCHQP and QCHKP were increased by PEF, whereas those of KCHKP and QCHQP were reduced. The basic structures and functional groups of peptides were unaffected. PEF treatment reduced the α-helix contents of KCHQP and QCHKP, but increased those of KCHKP and QCHQP. Moreover, the chemical shifts at 14.46 ppm, 8.22 ppm, 7.87 ppm, 7.24 ppm, and 6.13 ppm attributable to hydrogen atoms of QCHKP shifted to the right, but the active hydrogens of QCHQP were not affected by PEF.



中文翻译:

脉冲电场引起的二级结构变化影响松子(红松)蛋白五肽的抗氧化活性

我们使用脉冲电场(PEF)处理具有相似氨基酸序列(KCHKP,KCHQP,QCHKP和QCHQP)的四个五肽。然后,我们根据三价铁的还原抗氧化能力(FRAP)和清除羟自由基的能力评估了五肽的抗氧化活性。通过高效液相色谱(HPLC),中红外(MIR),圆二色性(CD)光谱和核磁共振(NMR)确定肽的结构。结果表明,PEF提高了KCHQP和QCHKP的抗氧化活性,而降低了KCHKP和QCHQP的抗氧化活性。肽的基本结构和官能团不受影响。PEF处理降低了KCHQP和QCHKP的α-螺旋含量,但增加了KCHKP和QCHQP的α-螺旋含量。此外,化学位移为14.46 ppm,8.22 ppm,7.87 ppm,7。

更新日期:2018-01-12
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