Objectives

Enzyme inhibitors minimize the degradation of unprotected collagen of dentin promoted by matrix metalloproteinases (MMPs) and cysteine cathepsins (CCs). As the evidence of their effect on the root canal is limited, this study aimed to evaluate the role of EDTA, chlorhexidine and E-64 as antiproteolytic agents on the bond strength (BS) of glass-fiber posts in root canals.

Materials and methods

Ninety-six bovine roots were distributed in groups for each time point (n = 8). Adper Scotchbond Multipurpose (MP)/ RelyX ARC system was used to lute the post according to the treatment: negative control (NC)- water, EDTA- 17% ethylenediaminetetraacetic acid, CHX- 2% digluconate chlorhexidine, E-64-5- 5 μM E-64, E-64-10- 10 μM E-64 and positive control (PC)- MP associated with activator/ catalyst. Then, slices were subjected to push-out test (0.5 mm/min) after 24 h/6 mons. Data were analyzed by three-way ANOVA/Tukey tests. Failure modes were analyzed (40×).

Results

The factors treatment, time, root canal third and the interaction between treatment and time were statistically significant. At 24 h, no negative interactions were observed among the root dentin, bonding system and post. At 6 mons, CHX improved the BS for middle and apical root thirds.

Conclusions

CHX was able to promote beneficial BS after 6 mons, which was not noted for any other tested enzyme inhibitors.

中文翻译：

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诱惑六个月后，蛋白水解抑制剂如何与玻璃纤维上的牙本质相互作用

目标

酶抑制剂可将基质金属蛋白酶（MMP）和半胱氨酸组织蛋白酶（CCs）促进的未保护牙本质胶原蛋白的降解降至最低。由于它们对根管的作用的证据有限，因此本研究旨在评估EDTA，洗必泰和E-64作为抗蛋白水解剂对根管中玻璃纤维桩的结合强度（BS）的作用。

材料和方法

在每个时间点（n = 8）成组分配了九十六个牛根。根据处理方法，使用Adper Scotchbond多功能（MP）/ RelyX ARC系统冲洗柱子：阴性对照（NC）-水，EDTA- 17％乙二胺四乙酸，CHX-2％二葡萄糖酸洗必太，E-64-5- 5 μME-64，E-64-10- 10μME-64和与活化剂/催化剂相关的阳性对照（PC）-MP。然后，在24小时/ 6个月后，对切片进行推出试验（0.5mm / min）。数据通过三向ANOVA / Tukey测试进行分析。分析了失效模式（40倍）。

结果

治疗的因素，时间，根管第三位以及治疗与时间之间的相互作用具有统计学意义。在24小时，在根部牙本质，结合系统和后部之间没有观察到负面的相互作用。在6个星期一时，CHX改善了中，根三分之二的BS。

结论

CHX能够在6个月后促进有益的BS，这对于任何其他测试的酶抑制剂均未发现。