Proton magnetic resonance spectroscopy (MRS) with optimized relaxation time is an effective method to quantify hepatic fatty acid values and characterize steatosis. The aim of this study is to quantify the difference in hepatic lipid content with metabolic changes during the progression of steatosis by using localized MRS sequence with T₂ relaxation time determination. Fatty liver disease was induced in C57BL/6N mice through a high-fat diet (HFD) of pellets containing 60% fat, 20% protein, and 20% carbohydrates. We used stimulated echo acquisition mode (repetition time: 3500 ms; mixing time: 10 ms; echo time: 20 ms) sequence. Using enhanced and mono exponential curve-fitting methods, the lipid relaxation time in mice was estimated at a fixed repetition time of 5000 ms and echo time ranging from 20 to 70 ms. The calculated lipid contents with incorrect and correct relaxation times were as follows: total saturated fatty acid (4.00 ± 2.90 vs 6.74 ± 2.25, p < 0.05 at week 0; 15.23 ± 9.94 vs 25.53 ± 10.49, p < 0.05 at week 4); total unsaturated fatty acid (0.40 ± 0.49 vs 0.56 ± 0.47, p < 0.05 at week 4; 0.33 ± 0.26 vs 0.60 ± 0.21, p < 0.01 at week 7); total unsaturated bond (0.48 ± 0.52 vs 1.05 ± 0.58, p < 0.05 at week 10). Furthermore, we determined that the correct relaxation times of triglycerides between 0 and 10 weeks were significantly altered in the resonances (∼2.03 ppm: 31.07 ± 1.00 vs 27.62 ± 1.20, p < 0.01; ∼2.25 ppm: 29.10 ± 1.52 vs 26.39 ± 1.08, p < 0.05; ∼2.78 ppm: 37.67 ± 2.92 vs 29.37 ± 2.64, p < 0.001). The work presented focused on the significance of the J-coupling effect. The selection of an appropriate relaxation time considering the J-coupling effect provides an effective method for quantifying lipid contents and characterizing hepatic steatosis.

具有优化弛豫时间的质子磁共振波谱（MRS）是量化肝脏脂肪酸值和表征脂肪变性的有效方法。这项研究的目的是通过使用带有T _2的局部MRS序列来量化脂肪变性过程中肝脂质含量与代谢变化之间的差异。弛豫时间的确定。通过高脂饮食（HFD）的含60％脂肪，20％蛋白质和20％碳水化合物的小球，可在C57BL / 6N小鼠中诱发脂肪肝疾病。我们使用受激回波采集模式（重复时间：3500毫秒；混合时间：10毫秒；回波时间：20毫秒）序列。使用增强和单指数曲线拟合方法，在固定的5000毫秒重复时间和20到70毫秒的回波时间下估计了小鼠的脂质松弛时间。正确和不正确的松弛时间计算出的脂质含量如下：总饱和脂肪酸（第0周时为4.00±2.90 vs 6.74±2.25，p <0.05；第4周时为15.23±9.94 vs 25.53±10.49，p <0.05）；总不饱和脂肪酸（第4周时为0.40±0.49 vs.0.56±0.47，p <0.05;第7周时为0.33±0.26 vs.0.60±0.21，p <0.01）; 总不饱和键（0.48±0.52 vs 1.05±0.58，第10周时p <0.05）。此外，我们确定在0至10周之间甘油三酸酯的正确弛豫时间在共振中有显着变化（〜2.03 ppm：31.07±1.00与27.62±1.20，p <0.01;〜2.25 ppm：〜2.25 ppm：29.10±1.52与26.39±1.08 ，p ＜0.05；〜2.78ppm：37.67±2.92对29.37±2.64，p ＜0.001）。提出的工作集中于J耦合效应的重要性。考虑J耦合效应选择合适的弛豫时间为定量脂质含量和表征肝脂肪变性提供了一种有效的方法。07±1.00对27.62±1.20，p <0.01; 约2.25 ppm：29.10±1.52对26.39±1.08，p <0.05; 约2.78 ppm：37.67±2.92对29.37±2.64，p <0.001）。提出的工作集中于J耦合效应的重要性。考虑J耦合效应选择合适的弛豫时间为定量脂质含量和表征肝脂肪变性提供了一种有效的方法。07±1.00对27.62±1.20，p <0.01; 约2.25 ppm：29.10±1.52对26.39±1.08，p <0.05; 约2.78 ppm：37.67±2.92对29.37±2.64，p <0.001）。提出的工作集中于J耦合效应的重要性。考虑J耦合效应选择合适的弛豫时间为定量脂质含量和表征肝脂肪变性提供了一种有效的方法。