A magnetic nanocomposite was prepared by entrapment of Fe₃O₄ nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization of cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme, and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structure of support was characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM, and DLS. The activity and stability of immobilized cellulase were investigated in the prepared support. The results showed that the ionic surface and covalent binding of enzyme onto the support improved the activity, thermal stability, and reusability of cellulase compared to free cellulase.

中文翻译：

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使用磁性聚离子液体支持物共价固定纤维素酶：提高酶活性和稳定性

通过截留Fe ₃ O ₄制备磁性纳米复合材料纳米粒子变成交联的离子液体/环氧树脂型聚合物。通过与环氧基的反应，将所得的载体用于纤维素酶的共价固定。载体的离子表面改善了酶的吸附，并且大量的酶（106.1 mg / g）被加载到载体表面上。还研究了离子单体的存在和酶的共价结合的影响。支撑物的结构通过各种仪器进行表征，例如FT-IR，TGA，VSM，XRD，TEM，SEM和DLS。在制备的载体中研究了固定化纤维素酶的活性和稳定性。结果表明，与游离纤维素酶相比，酶在载体上的离子表面和共价结合提高了纤维素酶的活性，热稳定性和可重复使用性。