Aflatoxin B₁ (AFB₁) causes hepatotoxic, genotoxic, and immunotoxic effects in a variety of species. Although various neutralizing agents of AFB₁ toxicity have been studied, the egg yolk immunoglobulin (IgY) detoxification of small molecular toxins and the mechanisms underlying such effects have not yet been reported. In this investigation, anti-AFB₁ IgY against AFB₁ was successfully raised, and a competitive indirect enzyme-linked immunosorbent assay was established with a sensitive half-maximal inhibitory concentration (IC_50, 2.4 ng/mL) and dynamic working range (0.13–43.0 ng/mL). The anti-AFB₁ IgY obtained reduced AFB₁-induced cytotoxicity, cellular dysfunction, and genotoxicity by protecting cells against apoptotic body formation and DNA strand breaks, preventing G2/M phase cell cycle arrest, reducing AFB₁-DNA adduct and reactive oxygen species production and maintaining cell migration and invasion and the mitochondrial membrane potential. Anti-AFB₁ IgY significantly inhibited the AFB₁-induced expression of proteins related to antioxidative, pro-apoptotic, and antiapoptotic processes in a strong dose-dependent manner. These experiments demonstrated that the anti-AFB₁ IgY-bound AFB₁ could not enter cells. This is the first time that IgY has been found to reduce the effects of small molecular toxins, which will be beneficial for the development of antibodies as detoxication agents.

中文翻译：

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IgY降低人L-02肝细胞和Swan 71滋养细胞中AFB ₁诱导的细胞毒性，细胞功能障碍和基因毒性。

黄曲霉毒素B ₁（AFB ₁）在多种物种中引起肝毒性，遗传毒性和免疫毒性。尽管已经研究了AFB ₁毒性的各种中和剂，但尚未报道蛋黄免疫球蛋白（IgY）对小分子毒素的解毒作用以及引起这种作用的机制。在该研究中，抗AFB ₁对抗AFB的IgY ₁被成功升高，并与一个敏感的半最大抑制浓度（IC建立了间接竞争酶联免疫吸附测定_50， 2.4毫微克/毫升）和动态工作范围（0.13 –43.0 ng / mL）。抗AFB ₁ IgY获得减少的AFB ₁保护细胞免受凋亡小体形成和DNA链断裂，防止G2 / M期细胞周期停滞，减少AFB ₁ -DNA加合物和活性氧生成并维持细胞迁移和侵袭以及线粒体的诱导的细胞毒性，细胞功能障碍和遗传毒性膜电位。抗AFB ₁ IgY以强烈的剂量依赖性方式显着抑制AFB ₁诱导的与抗氧化，促凋亡和抗凋亡过程相关的蛋白质表达。这些实验证明抗AFB ₁ IgY结合的AFB ₁无法进入牢房。这是首次发现IgY可以降低小分子毒素的作用，这将有利于抗体作为脱毒剂的开发。