Biorefining of bilberry pomace using innovative technologies such as microwave hydrodiffusion and gravity extraction (MHG) and ultrasound-assisted extraction (UAE) with different concentrations of ethanol/water as the solvent was established. Bead milling was also utilized in this sequential extraction scheme to remove the lipophilic fraction. The solubility index of target polyphenols was predicted using a computational tool (COSMO-RS) and compared to experimental results obtained by in vitro antioxidant activity assessments. MHG extracts obtained using microwave power of 2 W/g had the highest Folin–Ciocalteu reducing capacity (43.46 ± 0.48 mg gallic acid equivalent (GAE)/g of extract), total flavonoid (4.17 ± 0.04 mg quercetin equivalents (QE)/g of extract), total monomeric anthocyanin content (12.19 ± 0.13 mg delphinidin-3-O-glucoside equivalents (D3GE)/g of extract), and radical scavenging capacity (22.64 ± 2.23 mg trolox equivalents (TE)/g of extract). In UA ethanol/water extracts, the highest flavonoid (10.41 ± 0.08 mg QE/g of extract) and anthocyanin content (12.19 ± 0.51 D3GE mg/g of extract) was present in ethanol (100%), these results were in good correlation with computational predictions. The lipid fraction recovered from pomace using bead-milling extraction was mainly composed of oil rich in polyunsaturated linoleic and linolenic acids.

中文翻译：

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越橘（生物精炼的欧洲越桔L.）果渣微波Hydrodiffusion和重力，超声辅助，和珠磨提取

建立了以创新技术（如微波加氢扩散和重力萃取（MHG）和超声辅助萃取（UAE））以不同浓度的乙醇/水作为溶剂对越橘果渣进行生物精制的方法。在该连续提取方案中还使用了珠磨，以除去亲脂性级分。使用计算工具（COSMO-RS）预测目标多酚的溶解度指数，并将其与通过体外抗氧化剂活性评估获得的实验结果进行比较。使用2 W / g微波功率获得的MHG提取物具有最高的Folin–Ciocalteu还原能力（43.46±0.48 mg没食子酸当量（GAE）/ g提取物），总类黄酮（4.17±0.04 mg槲皮素当量（QE）/ g提取物），总单体花色苷含量（12.19±0.13 mg delphinidin-3-O-葡萄糖苷当量（D3GE）/克提取物）和自由基清除能力（22.64±2.23 mg trolox当量（TE）/克提取物）。在UA乙醇/水提取物中，乙醇（100％）中存在最高的类黄酮（10.41±0.08 mg QE / g提取物）和花青素含量（12.19±0.51 D3GE mg / g提取物），这些结果具有良好的相关性与计算预测。通过珠磨提取从果渣中回收的脂质部分主要由富含多不饱和亚油酸和亚麻酸的油组成。