A battery of cell-based bioassays, including PLHC-1 cells, zebrafish-Pxr-transfected COS-7 cells and estrogen receptor-recombinant yeast assay (ER-RYA), were applied to detect the presence of bioactive pollutants in sediments collected from Kaštela Bay and Brač Channel (Croatia). Exposure of PLHC-1 cells to the sediment extracts evidenced significant cytotoxicity and presence of CYP1A inducers in sediments collected in Kaštela Bay, near the industrial zone and cargo port of Split. Sediments from this area, which is highly contaminated with PCBs, HCB, DDTs and γ-HCH, also activated the zebrafish Pxr (zfPxr) reporter system. No evidence of estrogenicity was detected for any of the sediments extracts in the ER-RYA assay. Importantly, the battery of in vitro assays identified Kaštela Bay as the area with the higher anthropogenic impact, where sediment-bound pollutants could pose a risk to aquatic organisms. In contrast, sediments from the Brač Channel showed rather low response in the different bioassays.

一系列基于细胞的生物测定，包括PLHC-1细胞，斑马鱼Pxr转染的COS-7细胞和雌激素受体重组酵母测定（ER-RYA），用于检测从Kaštela收集的沉积物中的生物活性污染物的存在。海湾和布拉奇海峡（克罗地亚）。在沉积物提取物中暴露PLHC-1细胞证明在卡斯泰拉湾，工业区和斯普利特货运港口附近收集的沉积物中存在明显的细胞毒性和CYP1A诱导剂的存在。来自该地区的沉积物受到PCBs，HCB，DDT和γ-HCH的高度污染，也激活了斑马鱼Pxr（zfPxr）报告系统。在ER-RYA分析中，未检测到任何沉淀物提取物的雌激素性证据。重要的是，一系列体外试验将卡斯泰拉湾确定为具有更高人为影响的区域，与沉积物结合的污染物可能对水生生物构成危险。相反，在不同的生物测定中，布拉奇海峡的沉积物显示出相当低的响应。