Antibiotic residues in swine manure when entered the soil would most likely affect the complex composition and functions of the soil microbiome, which is also responsible for degrading these antibiotics. Three different methods of adding ciprofloxacin (CIP), a common antibiotic used in the swine industry, to the soil were used to investigate the effects of CIP on the soil microbiome and the degradation of CIP. Results of the study showed that the microbiome could promote the degradation of CIP in the soil when CIP was incorporated into the soil together with manure. However, the CIP degradation time was prolonged when adding the manure of swine fed with diet containing CIP in the soil. All treatments did not affect the copy number of the resistance genes, except for aac(6′)-Ib-cr, as compared with the initial numbers of each treatment. MiSeq Illumina sequencing and Biolog-ECO microplates results showed that CIP had a significant effect on the abundance, structure, and function of the soil microbiome, but different addition methods resulted in distinct effects. Results of the present study demonstrated that the microbiome and fate of CIP responded differently to the different methods of adding CIP to the soil.

猪粪中的抗生素残留物进入土壤后，很可能会影响土壤微生物组的复杂组成和功能，这也是导致这些抗生素降解的原因。通过三种不同的方法向土壤中添加环丙沙星（CIP）（一种用于养猪业的常用抗生素）来研究CIP对土壤微生物组和CIP降解的影响。研究结果表明，当将CIP与肥料一起掺入土壤时，微生物组可以促进CIP在土壤中的降解。但是，在土壤中添加饲喂含CIP饲料的猪粪时，CIP降解时间延长。除aac（6'）-Ib-cr外，所有处理均不影响抗性基因的拷贝数，与每种处理的初始编号相比。MiSeq Illumina测序和Biolog-ECO微孔板结果表明，CIP对土壤微生物组的丰度，结构和功能有显着影响，但不同的添加方法会产生不同的影响。本研究结果表明，CIP的微生物组和命运对向土壤中添加CIP的不同方法的反应不同。