This work was designed to utilize the integration of ultrasonic-microwave assisted preceded by enzymolysis pretreatment to enhance the extraction efficiency of essential oil (EO) from wild Lavandula pubescens (Lp). Cellulase (C-ase), hemicellulase (H-ase), and mixed enzymes (C-ase/H-ase) were used at 10 mg/100 mL of the liquid phase. Gas chromatography-mass spectrometry (GC-MS) system was used for chemical composition analyses of Lavandula pubescens essential oil (Lp-EO). C-ase pretreatment achieved the highest yield (0.50%) of EO and highest ratio of carvacrol (29.22%) as the main compound in comparison to other treatments. Changes in the morphological structure of Lp tissues were tracked using scanning electron microscopy (SEM). Free radical-scavenging activity was assessed using DPPH^•-SA and ABTS^•+-SA assays. The optimal extraction conditions were enzyme concentration of 14.82 mg/100 mL, sonication time of 38.18 min, and microwave power at 333 W. At these conditions, the maximum % yield, % DPPH^•-SA, and ABTS^•+SA were 0.67%, 65.40%, and 0.025 (as mM Trolox), respectively. Results exhibited that the isolated Lp-EO could be used as antifree radical and antibacterial agents in the biological systems.

中文翻译：

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集成的超声波-微波技术和酶促预处理增强薰衣草青春期释放精油的传质强化

这项工作旨在利用超声-微波辅助的整合，然后进行酶解预处理，以提高从野生薰衣草（Lp）提取精油（EO）的效率。以10 mg / 100 mL液相使用纤维素酶（C酶），半纤维素酶（H酶）和混合酶（C酶/ H酶）。气相色谱-质谱（GC-MS）系统用于毛for薰衣草的化学成分分析精油（Lp-EO）。与其他处理相比，C酶预处理可实现最高的EO收率（0.50％）和最高的香芹酚比率（29.22％）。使用扫描电子显微镜（SEM）跟踪Lp组织的形态结构变化。使用DPPH ^• -SA和ABTS ^•+ -SA分析评估清除自由基的活性。最佳提取条件为酶浓度为14.82 mg / 100 mL，超声处理时间为38.18 min，微波功率为333W。在这些条件下，最大％收率，％DPPH ^• -SA和ABTS ^•+SA分别为0.67％，65.40％和0.025（以mM Trolox计）。结果表明，分离出的Lp-EO可用作生物系统中的抗自由基和抗菌剂。