Zizania latifolia commonly known as wild rice grass which is available in huge quantities in Loktak Lake is a major concern as it occupies a large area of the Lake and causing a several environmental problems. The investigation of present study was to evaluate possibilities of using Zizania latifolia as feed stock for bioethanol production. The method involved the pretreatment with dilute acid or alkali followed by enzymatic hydrolysis with commercial cellulase. Acid pretreatment was performed with 10% biomass loading with different concentration of acids (0.4–2% w/v) and alkali (0.25–1.5% w/v). Maximum sugar release of 457 mg/g was obtained from 10% biomass loading and 2% w/v of acids. Alkali pretreatment is not effective for this grass. Physicochemical characterization of untreated and treated biomass was carried out by XRD, FTIR, SEM and corresponding alterations in the chemical composition were also monitored. Results showed the feasibility of this grass as biofuel (bioethanol) feed stock and can be potential approach to address the sustainable utilization phumdis grasses of Loktak Lake for the production of value added product.

人们普遍关注的iz子草（Zizania latifolia）在洛克塔克湖（Loktak Lake）有大量供应，因为它占据了大面积的湖水，并引起了许多环境问题。本研究的研究是为了评估使用Zizania latifolia的可能性作为生物乙醇生产的原料。该方法包括用稀酸或稀碱进行预处理，然后用商业纤维素酶进行酶促水解。酸预处理是在10％的生物量负载下进行的，其中酸（0.4–2％w / v）和碱（0.25–1.5％w / v）的浓度不同。从10％的生物量负载和2％w / v的酸中获得最大的457 mg / g糖释放。碱预处理对此草无效。通过XRD，FTIR，SEM对未处理和处理过的生物质进行理化表征，并监测化学成分的相应变化。结果表明，这种草作为生物燃料（生物乙醇）的原料是可行的，并且可能是解决Loktak湖可持续利用Phumdis草的增值产品生产的潜在途径。