Recalcitrance limits biomass application in biorefinery. It is even more so when toxic chlorophenols are present. In this study, we screened a microbial consortium, OEM2, for lignocellulose deconstruction and chlorophenols detoxification through a short-term and efficient screening process. Microbial consortium OEM2 had a good buffer capability in the cultivation process and exhibited a high xylanase activity, with over 85% hemicellulose degradation within 12 days. Throughout the treatment process, 41.5% rice straw decomposition on day12 and around 75% chlorophenols (MCP, 2,4-DCP, 2,4,6-TCP) removal on day 9, were recorded. Moreover, Fourier translation infrared spectroscopy (FTIR) analysis indicated that chemical bonds and groups (eg. hydrogen-bond, β-1,4 glycosidic bond, lignin-carbohydrate cross-linking) in the rice straw were broken. Cuticle and silica layer destruction and subsequent exposed cellulose fibers were observed by scanning electron microscopy (SEM). Microbial consortium OEM2 diversity analysis by 16S rRNA gene sequencing indicated that Proteobacteria (41.3%) was the most abundant phylum and the genera Paenibacillus and Pseudomonas played an important role in the lignocellulose decomposition and chlorophenols detoxification. This study developed a faster and more efficient strategy to screen a specific microbial consortium. And the new microbial consortium, OEM2, makes lignocellulose more accessible and complex pollutants unproblematic in the further biorefinery process.

顽固性限制了生物质在生物炼制中的应用。当存在有毒的氯酚时，情况更是如此。在这项研究中，我们通过短期有效的筛选过程筛选了微生物联盟OEM2，以进行木质纤维素的分解和氯酚的解毒。微生物联盟OEM2在培养过程中具有良好的缓冲能力，并表现出高的木聚糖酶活性，在12天内半纤维素降解率超过85％。在整个处理过程中，第12天记录了稻草分解的41.5％，第9天记录了约75％的氯酚（MCP，2,4-DCP，2,4,6-TCP）的去除。此外，傅立叶变换红外光谱（FTIR）分析表明稻草中的化学键和基团（例如氢键，β-1,4糖苷键，木质素-碳水化合物交联）被破坏。通过扫描电子显微镜（SEM）观察到表皮和二氧化硅层的破坏以及随后暴露的纤维素纤维。通过16S rRNA基因测序进行的微生物联盟OEM2多样性分析表明：变形杆菌（41.3％）是最丰富的门，Paenibacillus和Pseudomonas属在木质纤维素分解和氯酚解毒中起重要作用。这项研究开发了一种更快，更有效的策略来筛选特定的微生物联盟。而且，新的微生物联盟OEM2使木质纤维素更容易获得，并且在进一步的生物精炼过程中，复杂的污染物毫无问题。