Aptasensor for highly sensitive determination of aflatoxin M1 (AFM1) was developed on the base of glassy carbon electrode (GCE) covered with polymeric Neutral red (NR) dye obtained by electropolymerization in the presence of polycarboxylated pillar[5]arene derivative. Aptamer against AFM1 and NR label were then covalently linked to the carboxylic groups of the carrier by carbodiimide binding. At presence of AFM1 the cathodic peak current related to the NR conversion decreases. AFM1 induced also an increase of the charge transfer resistance measured by electrochemical impedance spectroscopy. In optimal conditions, this make it possible to determine from 5 to 120 ng/L AFM1 in standard solutions with limit of detection (LOD) of 0.5 ng/L. The aptasensor was validated on the spiked samples of cow and sheep milk as well as in kefir after their methanol dilution. Reliable detection of the 40–160 ng/kg of mycotoxins was reached. This is below limited threshold value (50 μg/kg) established in EC.

中文翻译：

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聚中性红和羧化柱[5]芳烃的电化学适应传感器灵敏测定黄曲霉毒素M1

在覆盖有聚合物中性红（NR）染料的玻璃碳电极（GCE）的基础上，开发了用于高灵敏度测定黄曲霉毒素M1的适应传感器，该电极通过在聚羧化柱[5]芳烃衍生物存在下进行电聚合而获得。然后通过碳二亚胺结合将抗AFM1和NR标记的适体共价连接到载体的羧基上。在存在AFM1时，与NR转换相关的阴极峰值电流减小。AFM1还引起通过电化学阻抗谱测量的电荷转移电阻的增加。在最佳条件下，这可以测定标准溶液中的5到120 ng / L AFM1，检测限（LOD）为0.5 ng / L。适配体传感器已在加标的牛，羊奶以及甲醇稀释后的开菲尔样品中得到验证。可以可靠地检测出40–160 ng / kg的霉菌毒素。这低于EC中设定的极限阈值（50μg/ kg）。