Trinitrobenzensulfonic acid (TNBS) is commonly used to induce an experimental inflammatory bowel disease (IBD) model. Oxidative stress and inflammation have been proposed as mechanisms underlying the pathophysiology of IBD. Aronia melanocarpa fruit juice (AMFJ) is extremely rich in polyphenolic substances, mainly proanthocyanidins, flavonoids and phenolic acids.

The aim of this study was to evaluate the effect of AMFJ in a rat TNBSinduced colitis model and to compare the effect of the juice with that of sulfasalazine.

Colitis was induced by TNBS in male Wistar rats. After the induction of colitis, AMFJ at three doses (2.5, 5 and 10 mL/kg) and sulfasalazine (400 mg/kg) were administered orally till the 14th experimental day. Severity of colitis was assessed by macroscopic and histopathological criteria. Oxidative stress was evaluated by the concentration of thiobarbituric acid reactive substances (TBARS).

TNBS caused severe colonic damage. AMFJ dose-dependently ameliorated TNBS-induced colitis. It improved the macroscopic and microscopic signs of colitis, and prevented the increase of colonic TBARS concentrations. Regarding different indices, the effect of AMFJ was comparable or even higher than that of sulfasalazine. In conclusion, the ameliorative effects of AMFJ in the experimental TNBSinduced colitis might be the result of its potent antioxidant and antiinflammatory properties.

三硝基苯磺酸（TNBS）通常用于诱导实验性炎症性肠病（IBD）模型。已经提出氧化应激和炎症是IBD的病理生理基础的机制。Aronia melanocarpa果汁（AMFJ）富含多酚类物质，主要是原花青素，类黄酮和酚酸。

这项研究的目的是评估AMFJ在大鼠TNBS诱导的结肠炎模型中的作用，并比较果汁与柳氮磺吡啶的作用。

TNBS在雄性Wistar大鼠中诱发结肠炎。诱发结肠炎后，口服给予三种剂量（2.5、5和10 mL / kg）的AMFJ和柳氮磺胺吡啶（400 mg / kg），直到实验第14天。通过宏观和组织病理学标准评估结肠炎的严重程度。通过硫代巴比妥酸反应性物质（TBARS）的浓度评估氧化应激。

TNBS造成了严重的结肠损伤。AMFJ剂量依赖性地改善了TNBS诱导的结肠炎。它改善了结肠炎的宏观和微观体征，并防止了结肠TBARS浓度的增加。关于不同的指标，AMFJ的作用与柳氮磺胺吡啶相当或什至更高。总之，AMFJ在实验性TNBS诱发的结肠炎中的改善作用可能是其有效的抗氧化剂和抗炎特性的结果。