We pioneer an approach for the visualization of pH-controlled molecular release of a fluorescent drug from a nitroxide polymer which combined yields a profluorescent system. A methacrylate-based terpolymer, bearing water-solubilizing triethylene-glycol chains, unpaired spins in the form of nitroxide and hydrazone-bound doxorubicin was synthesized to explore the possibilities of release quantification from profluorescent assemblies. Critically, we establish the fluorescence-silencing effect of polymer-bound nitroxides on covalently attached fluorophores and the overall accessibility of such constructs. The facile approach towards a functional assembly shown here provides an easy-to-assemble construct for complex delivery architectures towards applications in theranostics.

中文翻译：

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通过未配对的自旋荧光沉默 报告pH敏感药物的释放†

我们开创了一种可视化方法，可用于可视化从硝基氧化物聚合物中控制荧光药物的pH值控制的分子释放，这些方法共同产生了前荧光系统。合成了一种甲基丙烯酸酯基三元共聚物，其带有水溶性三乙二醇链，以氮氧化物形式存在的不成对的自旋和与-结合的阿霉素，以探索从荧光前体中释放定量的可能性。至关重要的是，我们建立了聚合物结合的氮氧化物对共价连接的荧光团的荧光沉默作用以及此类构建体的整体可及性。此处显示的用于功能组件的简便方法为复杂的交付体系结构提供了易于组装的构造，从而可用于诊断学中的应用。