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Structure characterization of two novel polysaccharides from Colocasia esculenta (taro) and a comparative study of their immunomodulatory activities
Journal of Functional Foods ( IF 5.6 ) Pub Date : 2018-01-10 , DOI: 10.1016/j.jff.2017.12.067
Huixian Li , Zhou Dong , Xiaojia Liu , Huamin Chen , Furao Lai , Mengmeng Zhang

Two fractions of non-starch polysaccharides termed TPS-1 and TPS-2 were sequentially purified from the corms of Colocasia esculenta. Structural characterizations of TPS-1 and TPS-2 were determined. The average molecular weight of TPS-1 and TPS-2 were estimated to be 10.502 and 10.191 kDa, respectively. TPS-1 consisted of Rha (8.5%), Xyl (0.73%), Glc (88.57%), and Gal (2.56%), while TPS-2 consisted of Rha (3.54%), Arf (1.6%), Glc (87.92%), and Gal (6.94%). The presence of glycosidic linkages in the two fractions of polysaccharides were identified and were significantly different. TPS-2 exhibited a higher immunomodulatory activity than TPS-1 through an immunostimulation assay promoting NO, TNF-α, and IL-6 production in RAW 264.7 cells. Furthermore, TPS-2 could significantly enhance the phagocytosis ability of macrophages. The membrane receptors of TPS-2 were identified to be TLR2, TLR4, GR, and SR. Taken together, TPS-1 and TPS-2 could be considered as novel potential immunostimulants of food and pharmaceutical supplements for hypoimmunity.



中文翻译:

香芋(Colocasia esculenta(taro))的两种新型多糖的结构表征及其免疫调节活性的比较研究

Colocasia esculenta的球茎中依次纯化出两部分分别称为TPS-1和TPS-2的非淀粉多糖。。确定了TPS-1和TPS-2的结构特征。TPS-1和TPS-2的平均分子量分别估计为10.502和10.191 kDa。TPS-1由Rha(8.5%),二甲苯(0.73%),Glc(88.57%)和Gal(2.56%)组成,而TPS-2由Rha(3.54%),Arf(1.6%),Glc( 87.92%)和Gal(6.94%)。鉴定了多糖的两个部分中糖苷键的存在,并且存在显着差异。通过促进RAW 264.7细胞中NO,TNF-α和IL-6产生的免疫刺激试验,TPS-2表现出比TPS-1更高的免疫调节活性。此外,TPS-2可以显着增强巨噬细胞的吞噬能力。TPS-2的膜受体被鉴定为TLR2,TLR4,GR和SR。在一起

更新日期:2018-01-10
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