Abstract In this paper, the physio-chemical characteristics of green synthesised PLA/silver nanoparticles (PLA-AgNPs) system was investigated for H 2 O 2 sensing. The precursor, silver nitrate (AgNO 3 ) was reduced to nanosilver (n-Ag) by Piper nigrum leaves extract in dual phase medium containing PLA (Capping agent). X-ray photo electron spectroscopy (XPS) analysis was carried out to evaluate the interaction of PLA with silver nanoparticles. The morphological analysis by Transmission electron microscopy (TEM) obtained images of spherical silver particles with average size of about 10–20 nm. The H 2 O 2 sensing was confirmed from the colour fading of the solution and vanishing of the characteristic surface plasmon resonance (SPR) absorbance peak of PLA-AgNPs on adding H 2 O 2 . The proposed sensor can detect even very low concentrations (5 μM) of hydrogen peroxide and finds applicability in biological and environmental analysis.

中文翻译：

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用于检测生物样品中过氧化氢的绿色合成 PLA/银纳米粒子探针

摘要 本文研究了绿色合成PLA/银纳米粒子(PLA-AgNPs) 体系的理化特性，用于H 2 O 2 传感。在含有PLA（封端剂）的双相介质中，黑胡椒叶提取物将前体硝酸银（AgNO 3 ）还原为纳米银（n-Ag）。进行 X 射线光电子能谱 (XPS) 分析以评估 PLA 与银纳米粒子的相互作用。透射电子显微镜 (TEM) 的形态分析获得了平均尺寸约为 10-20 nm 的球形银颗粒的图像。H 2 O 2 传感通过溶液的褪色和加入 H 2 O 2 后 PLA-AgNPs 的特征表面等离子体共振 (SPR) 吸收峰的消失得到证实。