Chlorpyrifos (chl) is an organophosphate pesticide extensively used in agriculture and highly toxic for human health. Fluorine doped tin-oxide (FTO) based electrochemical nanosensor was developed for chlorpyrifos detection with gold nanoparticles (AuNPs) and anti-chlorpyrifos antibodies (chl-Ab). AuNPs provides high electrical conductivity and specific resistivity, thus increases the sensitivity of immunoassay. High electrical conductivity of AuNPs reveals that it promotes the redox reaction for better cyclic voltammetry. Based on the intrinsic conductive properties of FTO-AuNPs complex, chl-Ab was immobilized onto AuNPs surface. Under optimized conditions, the proposed FTO based nanosensor exhibited high sensitivity and stable response for the detection of chlorpyrifos, ranging from 1 fM to 1 µM with limit of detection (LOD) up to 10 fM. The FTO-AuNPs sensor was successfully employed for the detection of chlorpyrifos in standard as well in real samples up to 10 nM for apple and cabbage, 50 nM for pomegranate. The proposed FTO-AuNPs nanosensor can be used as a quantitative tool for rapid, on-site detection of chlorpyrifos traces in real samples when miniaturized due to its excellent stability, sensitivity, and simplicity.

毒死rif（chl）是一种有机磷酸盐农药，广泛用于农业，对人体健康具有剧毒。开发了基于氟掺杂氧化锡（FTO）的电化学纳米传感器，用于用金纳米颗粒（AuNPs）和抗毒死py抗体（ch1-Ab）进行毒死rif检测。AuNPs具有很高的电导率和比电阻，因此提高了免疫测定的灵敏度。AuNPs的高电导率表明它可以促进氧化还原反应，从而实现更好的循环伏安法。基于FTO-AuNPs复合物的固有导电特性，将chl-Ab固定在AuNPs表面。在最佳条件下，拟议中的基于FTO的纳米传感器对毒死rif的检测具有很高的灵敏度和稳定的响应，范围从1 fM到1 µM，检测限（LOD）高达10 fM。FTO-AuNPs传感器已成功用于检测标准品中的毒死rif，以及苹果和卷心菜中高达10 nM的石榴中的真实样品中的毒死rif。所提出的FTO-AuNPs纳米传感器由于其出色的稳定性，灵敏度和简便性，可在小型化时用作定量现场快速检测现场毒死rif痕迹的定量工具。