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Graphene oxide supported liposomes for efficient label free electrochemical DNA biosensing
Sensors and Actuators B: Chemical ( IF 8.4 ) Pub Date : 2018-01-09 , DOI: 10.1016/j.snb.2018.01.103
Habibulla Imran , Palinci Nagarajan Manikandan , Venkataraman Dharuman

Liposome and graphene are studied towards the development of biocompatible materials for DNA sensing and target delivery applications. In this study, liposomes are supported on various graphene materials on gold surface by physisorption without using thiol self-assembled monolayers. Reduced graphene oxides (rGO) are prepared by chemical (ascorbic acid and hydrazine) and electrochemical reduction methods. Studies indicate that the bilayer formation of cationic 1,2-dioleoyltrimethylammoniumpropane (DOTAP) on the graphite, graphene, graphene oxide and chemically reduced GO is due to thick layers of these materials. The thin layered rGO produced through mild electrochemical reduction in the acidic medium (favors selective reduction of hydroxyl and epoxide functional group) is suitable for the tethering intact vesicle structure of the DOTAP confirmed by different surface characterizing techniques. The intact vesicle structure is applied for label free sensing of DNA in the dynamic range from 10−15 to 10−9 M to demonstrate the feasibility of the sensor for molecular diagnosis.



中文翻译:

氧化石墨烯负载的脂质体可实现高效无标记的电化学DNA生物传感

研究了脂质体和石墨烯,以开发用于DNA传感和目标递送应用的生物相容性材料。在这项研究中,脂质体通过物理吸附作用负载在金表面的各种石墨烯材料上,而无需使用硫醇自组装单层膜。还原的氧化石墨烯(rGO)是通过化学方法(抗坏血酸和肼)和电化学还原方法制备的。研究表明,在石墨,石墨烯,氧化石墨烯和化学还原的GO上形成双层阳离子1,2-二油酰基三甲基铵丙烷(DOTAP)的原因是这些材料的厚层。通过在酸性介质中进行温和的电化学还原(有利于羟基和环氧基官能团的选择性还原)制得的薄层rGO适合于通过不同表面表征技术确认的DOTAP束缚完整囊泡结构。完整的囊泡结构可用于从10到10的动态范围内的DNA的无标记传感-15至10 -9  M,以证明该传感器用于分子诊断的可行性。

更新日期:2018-01-09
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