We report on the label-free glucose detection over a wide dynamic range by mesoporous Si nanowires (NWs) through its photoluminescence (PL) quenching and anomalous PL enhancement caused by glucose (GL) in presence of its conjugate enzyme glucose oxidase (GOx). The GL and GOx induced surface evolution of Si NWs is examined by several spectroscopic tools. At a low concentration of GL, the visible-NIR PL emission from Si NWs is partially quenched due to the electron removal by the H₂O₂ produced by the GL/GOx solution. At higher concentration of GL, the SiH bonds on the surface of the Si NWs are eliminated and different functional groups, e.g., CC, CC, COH, CO and COOH are attached. This makes the Si NWs highly photoluminescent as compared to the bare Si NWs. The linear response of the PL enhancement of Si NWs with the logarithm of GL concentration is utilized for the label free sensitive detection of GL. A high dynamic range (0.1–50 mM) for GL detection and a considerable lower limit of detection (1.06 μM) are achieved for the newly developed biosensor. This report opens up possibilities for exploiting the low cost mesoporous Si NW arrays for ultrasensitive biosensing and bioimaging applications.

我们报告了介孔硅纳米线（NWs）通过其光致发光（PL）猝灭和由葡萄糖（GL）在其共轭酶葡萄糖氧化酶（GOx）的存在下引起的异常PL增强而在宽动态范围内进行的无标记葡萄糖检测。GL和GOx诱导的Si NWs的表面演变已通过几种光谱仪进行了检查。在低的GL浓度下，由于GL / GOx溶液产生的H ₂ O ₂去除了电子，Si NWs的可见NIR PL发射被部分淬灭。在较高的GL浓度下，Si NW的表面上的Si H键被消除，并形成不同的官能团，例如C C，C C，C OH，CO和COOH连接。与裸Si NW相比，这使得Si NW具有高光致发光性。Si NWs的PL增强与GL浓度的对数之间的线性响应用于GL的无标记灵敏检测。新开发的生物传感器实现了用于GL检测的高动态范围（0.1–50 mM）和相当低的检测下限（1.06μM）。该报告为超低成本生物传感和生物成像应用开发低成本介孔Si NW阵列开辟了可能性。