A fluorogenic probe based on the novel fluorophore 5-triazole-quinoline was developed for the detection of hydrogen sulfide, an endogenous signaling molecule associated with the development of various diseases. The lysosome-targeted probe Lyso-HS was synthesized via C–H direct azidation from 8-aminoquinoline; it was able to detect H₂S in 1 min and exhibited excellent turn-on ability with 95-fold fluorescence enhancement based on a new fluorochrome. The high quenching efficiency was further verified using time-dependent density functional theory (TDDFT). The probe also exhibited high selectivity and a low detection limit (as low as 214.5 nM), which has practical applications for disease detection and monitoring.

中文翻译：

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一种基于5-三唑-喹啉的新型溶酶体荧光探针，可快速检测活细胞中的硫化氢†

开发了一种基于新型荧光团5-三唑-喹啉的荧光探针，用于检测硫化氢，硫化氢是一种与各种疾病发展相关的内源性信号分子。通过C–H直接叠氮化从8-氨基喹啉合成靶向溶酶体的探针Lyso-HS。它能够在1分钟内检测到H ₂ S，并且具有基于新荧光染料的95倍荧光增强的优异开启能力。使用时变密度泛函理论（TDDFT）进一步验证了高淬灭效率。该探针还显示出高选择性和低检测限（低至214.5 nM），在疾病检测和监测中具有实际应用。