The complexity of polymer–protein interactions makes rational design of the best polymer architecture for any given biointerface extremely challenging, and the high throughput synthesis and screening of polymers has emerged as an attractive alternative. A porphyrin‐catalysed photoinduced electron/energy transfer–reversible addition‐fragmentation chain‐transfer (PET‐RAFT) polymerisation was adapted to enable high throughput synthesis of complex polymer architectures in dimethyl sulfoxide (DMSO) on low‐volume well plates in the presence of air. The polymerisation system shows remarkable oxygen tolerance, and excellent control of functional 3‐ and 4‐arm star polymers. We then apply this method to investigate the effect of polymer structure on protein binding, in this case to the lectin concanavalin A (ConA). Such an approach could be applied to screen the structure–activity relationships for any number of polymer–protein interactions.

中文翻译：

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用于筛选构效关系的耐氧 PET-RAFT 聚合

聚合物-蛋白质相互作用的复杂性使得为任何给定生物界面合理设计最佳聚合物结构都极具挑战性，而聚合物的高通量合成和筛选已成为一种有吸引力的替代方案。采用卟啉催化的光诱导电子/能量转移-可逆加成断裂链转移（PET-RAFT）聚合，能够在存在以下物质的情况下在低体积孔板上高通量合成二甲亚砜（DMSO）中的复杂聚合物结构空气。该聚合体系表现出卓越的耐氧性，并对功能性 3 臂和 4 臂星形聚合物具有出色的控制能力。然后，我们应用这种方法来研究聚合物结构对蛋白质结合的影响，在本例中是凝集素伴刀豆球蛋白 A (ConA)。这种方法可用于筛选任意数量的聚合物-蛋白质相互作用的结构-活性关系。