Exercise stimulates the release of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognizing that many circulating proteins are packaged in extracellular vesicles (EVs), we employed quantitative proteomic techniques to characterize the exercise-induced secretion of EV-contained proteins. Following a 1-hr bout of cycling exercise in healthy humans, we observed an increase in the circulation of over 300 proteins, with a notable enrichment of several classes of proteins that compose exosomes and small vesicles. Pulse-chase and intravital imaging experiments suggested EVs liberated by exercise have a propensity to localize in the liver and can transfer their protein cargo. Moreover, by employing arteriovenous balance studies across the contracting human limb, we identified several novel candidate myokines, released into circulation independently of classical secretion. These data identify a new paradigm by which tissue crosstalk during exercise can exert systemic biological effects.

中文翻译：

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细胞外囊泡为运动过程中的组织串扰提供了一种手段。

运动会刺激分子释放到循环系统中，从而支持组织间信号蛋白是适应运动的重要介体的概念。认识到许多循环蛋白包装在细胞外囊泡（EVs）中，我们采用定量蛋白质组学技术来表征运动引起的EV包含蛋白的分泌。在健康人中进行1小时的循环运动后，我们观察到300多种蛋白质的循环增加，并且显着富集了组成外泌体和小囊泡的几种蛋白质。脉搏追踪和活体内成像实验表明，通过运动释放的电动车倾向于在肝脏中定位，并可以转移其蛋白质货物。而且，通过在整个人的收缩肢体中进行动静脉平衡研究，我们确定了几种新颖的候选肌动蛋白，它们独立于经典分泌物而释放到循环中。这些数据确定了运动过程中组织串扰可以发挥全身生物学效应的新范式。