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A comprehensive Caenorhabditis elegans N-glycan shotgun array
Glycobiology ( IF 4.3 ) Pub Date : 2018-01-09 , DOI: 10.1093/glycob/cwy002
Ewa Jankowska 1 , Lisa M Parsons 1 , Xuezheng Song 2 , Dave F Smith 2 , Richard D Cummings 3 , John F Cipollo 1
Affiliation  

Here we present a Caenorhabditis elegans N-glycan shotgun array. This nematode serves as a model organism for many areas of biology including but not limited to tissue development, host–pathogen interactions, innate immunity, and genetics. Caenorhabditis elegans N-glycans contain structural motifs that are also found in other nematodes as well as trematodes and lepidopteran species. Glycan binding toxins that interact with C. elegans glycoconjugates also do so with some agriculturally relevant species, such as Haemonchus contortus, Ascaris suum, Oesophagostomum dentatum and Trichoplusia ni. This situation implies that protein–carbohydrate interactions seen with C. elegans glycans may also occur in other species with related glycan structures. Therefore, this array may be useful to study these relationships in other nematodes as well as trematode and insect species. The array contains 134 distinct glycomers spanning a wide range of C. elegans N-glycans including the subclasses high mannose, pauci mannose, high fucose, mammalian-like complex and phosphorylcholine substituted forms. The glycans presented on the array have been characterized by two-dimensional separation, ion trap mass spectrometry, and lectin affinity. High fucose glycans were well represented and contain many novel core structures found in C. elegans as well as other species. This array should serve as an investigative platform for carbohydrate binding proteins that interact with N-glycans of C. elegans and over a range of organisms that contain glycan motifs conserved with this nematode.

中文翻译:

全面的秀丽隐杆线虫N-聚糖shot弹枪阵列

在这里,我们提出了秀丽隐杆线虫N-聚糖shot弹枪阵列。该线虫可作为许多生物学领域的模型生物,包括但不限于组织发育,宿主与病原体的相互作用,先天免疫和遗传学。秀丽隐杆线虫N-聚糖含有在其他线虫以及吸虫和鳞翅目物种中也发现的结构基序。与秀丽隐杆线虫糖缀合物相互作用的聚糖结合毒素也与一些与农业有关的物种相互作用,例如捻转血矛线虫Haemonchus contortus),AAscaris suum)牙食道齿牙and (Oesophagostomum dentatum)滴虫Trichoplusia ni)。。这种情况表明,与秀丽隐杆线虫聚糖发生的蛋白质-碳水化合物相互作用也可能发生在具有相关聚糖结构的其他物种中。因此,该阵列对于研究其他线虫以及吸虫和昆虫物种中的这些关系可能是有用的。该阵列包含134种不同的糖聚物,跨越广泛的秀丽隐杆线虫N-聚糖,包括高甘露糖,低聚甘露糖,高岩藻糖,哺乳动物样复合物和磷酰胆碱取代形式的亚类。通过二维分离,离子阱质谱和凝集素亲和力表征了阵列上存在的聚糖。高岩藻糖聚糖被很好地代表,并包含秀丽隐杆线虫中发现的许多新颖的核心结构以及其他物种。该阵列应作为与线虫的N-聚糖以及一系列含有该线虫保守的聚糖基序的生物体相互作用的碳水化合物结合蛋白的研究平台。
更新日期:2018-01-09
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