Abstract Here, an electrochemiluminescent aptasensor based on β-cyclodextrin/graphitic carbon nitride (β-CD/g-C3N4) composite for selective and highly ultrasensitive assay of platelet derived growth factor BB (PDGF-BB) is fabricated. To achieve the sensitive and specific detection of PDGF-BB, it is an available strategy to explore excellent electrochemiluminescent (ECL) luminophores with the advantages of high electrochemical-driven luminescent activity and a specific recognition function. In this work, for the first time a novel β-CD non-covalently functionalized g-C3N4 composite β-CD/g-C3N4 was employed to construct the ECL detection platform for PDGF-BB based on its aptamer. The limit of detection was determined to be as low as 2.6 × 10−13 g/mL. It was observed that β-CD was playing a key role in improving the adamantane-labeled DNA amount modified on the electrode surface. It can further help to amplify the ECL quenching effect by both energy transfer and photo-excited electron transfer processes between g-C3N4 emitter and ferrocene quencher. It is anticipated that β-CD/g-C3N4 may be a class of promising material for constructing ECL sensors.

中文翻译：

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基于β-环糊精/石墨氮化碳复合材料的电化学发光适体传感器用于血小板衍生生长因子BB的高选择性和超灵敏测定

摘要 在此，制备了一种基于 β-环糊精/石墨氮化碳 (β-CD/g-C3N4) 复合材料的电化学发光适体传感器，用于血小板衍生生长因子 BB (PDGF-BB) 的选择性和高灵敏度测定。为了实现PDGF-BB的灵敏和特异性检测，探索具有高电化学驱动发光活性和特异性识别功能的优异电化学发光（ECL）发光团是一种可行的策略。在这项工作中，首次采用新型 β-CD 非共价功能化 g-C3N4 复合物 β-CD/g-C3N4 构建了基于其适配体的 PDGF-BB 的 ECL 检测平台。检测限低至 2.6 × 10−13 g/mL。据观察，β-CD 在提高电极表面修饰的金刚烷标记 DNA 量方面起着关键作用。通过 g-C3N4 发射体和二茂铁猝灭剂之间的能量转移和光激发电子转移过程，它可以进一步帮助放大 ECL 猝灭效应。预计 β-CD/g-C3N4 可能是一类有前景的用于构建 ECL 传感器的材料。