Journal of the American Society for Mass Spectrometry ( IF 3.2 ) Pub Date : 2018-01-08 , DOI: 10.1007/s13361-017-1867-9 Matthew B. O’Rourke 1, 2 , Benjamin B. A. Raymond 3 , Steven P. Djordjevic 3 , Matthew P. Padula 2
Tissue imaging using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a well-established technique that, in recent years, has seen wider adoption and novel application. Applications such imaging mass spectrometry (IMS) and biotyping are beginning to gain greater exposure and use; however, with limitations in optimization methods, producing the best result often relies on the ability to customize the physical characteristics of the instrumentation, a task that is challenging for most mass spectrometry laboratories. With this in mind, we have described the effect of making simple adjustments to the laser optics at the final collimating lens area, to adjust the laser beam size and shape in order to allow greater customization of the instrument for improving techniques such as IMS. We have therefore been able to demonstrate that improvements can be made without requiring the help of an electrical engineer or external funding in a way that only costs a small amount of time.
中文翻译:
矩阵辅助激光解吸/电离质谱(MALDI-MS)中准直透镜聚焦对激光束形状的影响
使用基质辅助激光解吸/电离质谱(MALDI-MS)进行组织成像是一项行之有效的技术,近年来,这种技术已得到更广泛的采用和新颖的应用。成像质谱(IMS)和生物分型等应用开始获得更多的曝光和使用。然而,由于优化方法的局限性,产生最佳结果通常取决于定制仪器物理特性的能力,这对大多数质谱实验室来说都是一项艰巨的任务。考虑到这一点,我们描述了在最终的准直透镜区域对激光光学器件进行简单调整,调整激光束的大小和形状以允许对仪器进行更大程度定制以改进诸如IMS之类的技术的效果。