We report the synthesis of new polymer-protein conjugates using a grafting-from method employing photoinduced electron transfer reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Lipase, an industrially important enzyme, was modified with a reversible addition-fragmentation (RAFT) chain transfer agent (CTA) using activated ester chemistry. To study the effects of polymer composition and molecular weight on the enzyme activity, a hydrophilic and hydrophobic polymer were grafted at three different polymer lengths. We found a significant impact of polymer composition on enzyme activity with hydrophobic polymer substantially increasing enzyme activity.

我们报告了使用光诱导的电子转移可逆加成-断裂链转移（PET-RAFT）聚合反应的接枝方法合成新的聚合物-蛋白质共轭物。脂酶是一种工业上重要的酶，使用可活化的酯化学方法通过可逆的加成片段化（RAFT）链转移剂（CTA）进行了修饰。为了研究聚合物组成和分子量对酶活性的影响，以三种不同的聚合物长度接枝了亲水性和疏水性聚合物。我们发现聚合物组成对酶活性的显着影响，而疏水聚合物大大增加了酶活性。