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Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro
Biomacromolecules ( IF 6.2 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1021/acs.biomac.7b01492
Xiaoyan Wang 1, 2 , Jing Guan 1 , Xupin Zhuang 2 , Zhihong Li 1 , Shujie Huang 1 , Jian Yang 1 , Changjun Liu 1 , Fan Li 1 , Feng Tian 1 , Jimin Wu 1 , Zhan Shu 1
Affiliation  

N-Alkylated chitosan (NACS) may improve the blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACSs with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-ray diffraction (XRD). The corresponding NACS nanofiber membranes (NACS-NM) were subsequently fabricated by electronic spinning technique. SEM, XRD, DSC, surface area, porosity, contact angle, blood absorption, and mechanical properties were used to characterize the CS-NM/NACS-NM. Moreover, cytotoxicity, coagulation, activated partial thromboplastin time, plasma prothrombin time, thrombin time, and platelet aggregation tests were performed to evaluate the biocompatibility and blood coagulation properties of NACS-NM. The results showed that NACS-NM was not cytotoxic. NACS-NM with DS of 19.25% for N-hexane CS (CS6b), 17.87% for N-dodecane CS (CS12b), and 8.97% for N-octadecane CS (CS18a) exhibited good blood clotting performance. Moreover, NACS-NMs favored the activation of coagulation factors and platelets. In addition, intracellular Ca2+ was not related to platelet activation. The above results suggested that NACS-NM would be an effective hemostatic agent.

中文翻译:

N-烷基壳聚糖纳米纤维膜的体外凝血研究

ñ-烷基化壳聚糖(NACS)可以提高壳聚糖(CS)的血液凝结效率。为了研究其凝血能力,合成了一系列具有不同碳链长度和烷基取代度(DS)的NACS,并通过FTIR,NMR,元素分析和X射线衍射(XRD)对其进行了表征。随后通过电子纺丝技术制备了相应的NACS纳米纤维膜(NACS-NM)。SEM,XRD,DSC,表面积,孔隙率,接触角,血液吸收和机械性能用于表征CS-NM / NACS-NM。此外,进行了细胞毒性,凝血,活化的部分凝血活酶时间,血浆凝血酶原时间,凝血酶时间和血小板凝集试验,以评估NACS-NM的生物相容性和凝血特性。结果表明,NACS-NM没有细胞毒性。NACS-NM,DS为19.25%N-己烷CS(CS6b),N-十二烷CS(CS12b)占17.87%,N-十八烷CS(CS18a)占8.97%,具有良好的凝血性能。此外,NACS-NMs支持凝血因子和血小板的活化。另外,细胞内Ca 2+与血小板活化无关。上述结果表明,NACS-NM将是有效的止血剂。
更新日期:2018-01-08
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