Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a fast analysis tool for the detection of a wide range of analytes. However, heterogeneous distribution of matrix/analyte cocrystal, variation in signal intensity and poor experimental reproducibility at different locations of the same spot means difficulty in quantitative analysis. In this work, carbon nanotubes (CNTs) were employed as adsorbent for analyte cum matrix on a conductive porous membrane as a novel mass target plate. The sample pretreatment step was achieved by enrichment and dead-end filtration and dried by a solid-liquid separation. This approach enables the homogeneous distribution of analyte in the matrix, good shot-to-shot reproducibility in signals and quantitative detection of peptide and protein at different concentrations with correlation coefficient (R²) of 0.9920 and 0.9909, respectively. The simple preparation of sample in a short time, uniform distribution of analyte, easy quantitative detection, and high reproducibility makes this technique useful and may diversify the application of MALDI-MS for quantitative detection of a variety of proteins.

基质辅助激光解吸/电离质谱（MALDI-MS）是用于检测多种分析物的快速分析工具。然而，基质/分析物共晶的异质分布，信号强度的变化以及同一点不同位置的实验重现性较差，这意味着难以进行定量分析。在这项工作中，碳纳米管（CNTs）被用作新型多孔质靶板在导电多孔膜上的分析物和基质的吸附剂。样品的预处理步骤通过富集和末端过滤实现，并通过固液分离进行干燥。这种方法可以使分析物均匀分布在基质中，²）分别为0.9920和0.9909。短时间内样品的简单制备，分析物的均匀分布，易于定量检测和高重现性使该技术有用，并且可以使MALDI-MS在定量检测各种蛋白质上的应用多样化。