Changes in temperature, ionic strength or electrical field are generally employed to dehybridize double‐stranded DNA (dsDNA). In contrast, we propose potential‐induced dsDNA dehybridization to distinguish fully matched target DNA (tDNA) from tDNA with a single nucleotide polymorphism (SNP) by following their dehybridization kinetics through in situ surface enhanced Raman scattering (SERS). The determination of the potential that evokes notable dehybridization of dsDNA without causing any desorption of the immobilized probe DNA (pDNA) from the electrode surface was performed by investigating the desorption kinetics of labelled single‐stranded DNA (ssDNA) and dehybridization kinetics of dsDNA with labelled tDNA. This comparatively simple approach allows for SNP detection within minutes.

中文翻译：

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使用原位表面增强拉曼散射监测单核苷酸多态性检测的潜在诱导DNA脱氢动力学

温度，离子强度或电场的变化通常用于双链DNA（dsDNA）的去杂化。相比之下，我们提出了潜在诱导的dsDNA脱杂作用，通过遵循原位表面增强拉曼散射（SERS）的去杂化动力学，将具有单核苷酸多态性（SNP）的完全匹配的靶DNA（tDNA）与tDNA区分开。通过研究标记的单链DNA（ssDNA）的解吸动力学和带标记的dsDNA的去杂化动力学来确定在不引起固定化探针DNA（pDNA）从电极表面解吸的情况下引起dsDNA显着去杂化的潜力。脱氧核糖核酸 这种相对简单的方法可以在数分钟内完成SNP检测。