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Electrospinning: a carbonized gold/graphene/PAN nanofiber for high performance biosensing†
Analytical Methods ( IF 3.1 ) Pub Date : 2018-01-08 00:00:00 , DOI: 10.1039/c7ay02880f
Pongpol Ekabutr 1, 2, 3, 4 , Wimonwan Klinkajon 1, 2, 3, 4 , Pakakrong Sangsanoh 1, 2, 3, 4 , Orawan Chailapakul 2, 3, 5, 6, 7 , Pimolpan Niamlang 4, 8, 9, 10 , Thitikan Khampieng 4, 10, 11, 12, 13 , Pitt Supaphol 1, 2, 3, 4
Affiliation  

In this study, a novel biosensor was produced from a carbonized gold (Au)/graphene (G) hybrid nanowire fabricated on a disposable screen-printed carbon electrode (SPCE) in order to amplify signals. The processes of carbonization and electrospinning were merged in order to determine dopamine (DA) in a selective and sensitive manner when it is present in uric acid (UA) or ascorbic acid (AA). In order to assess the nature of the surface morphology and the physical product properties, transmission electron microscopy, scanning electron microscopy, and X-ray diffraction were employed prior to and following the carbonization step. The modified electrode (CPAN-Au/G) in [Fe(CN)6]3−/4− and DA was examined using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) in order to establish the electrochemical behavior. The findings from this test revealed that the modified electrode was capable of selectively identifying DA in 0.1 M PBS at pH 7.4 when other substances were present to provide interference. This process did not require the use of an anionic surfactant to serve as the discriminating agent. From the analysis of the DPV current, a linear dependence upon DA concentrations became apparent, in the range of 0.001–60 μM when the detection limit was 0.8 nM (S/N = 3) and sensitivity was 1.4351 μA cm−2. DA quantities in human serum could be effectively detected using the CPAN-Au/G electrode, and the modified electrode raises considerable expectations for application as a high-potential electrode. This would permit the determination of DA in a selective and sensitive manner while achieving sensor stability over the long term.

中文翻译:

静电纺丝:碳化的金/石墨烯/ PAN纳米纤维,用于高性能生物传感

在这项研究中,一种新颖的生物传感器是由在一次性丝网印刷碳电极(SPCE)上制造的碳化金(Au)/石墨烯(G)杂化纳米线制成的,目的是放大信号。合并碳化和静电纺丝过程,以便在尿酸(UA)或抗坏血酸(AA)中存在多巴胺(DA)时,能够以选择性和灵敏的方式确定多巴胺(DA)。为了评估表面形态的性质和物理产物的性质,在碳化步骤之前和之后使用透射电子显微镜,扫描电子显微镜和X射线衍射。[Fe(CN)6 ] 3-/- 4--中的修饰电极(CPAN-Au / G)为了确定电化学行为,使用循环伏安法(CV)和微分脉冲伏安法(DPV)检查了DA。该测试的结果表明,当存在其他物质以提供干扰时,修饰的电极能够在pH 7.4的0.1 M PBS中选择性鉴定DA。该方法不需要使用阴离子表面活性剂作为鉴别剂。根据DPV电流的分析,当检出限为0.8 nM(S / N = 3)且灵敏度为1.4351μAcm -2时,对DA浓度的线性依赖性变得明显,范围为0.001–60μM。。使用CPAN-Au / G电极可以有效地检测人血清中的DA量,而修饰电极对用作高电势电极提出了很高的期望。这将允许以选择性和灵敏的方式确定DA,同时实现长期的传感器稳定性。
更新日期:2018-01-08
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