Quercetin and its glycosides possess potential benefits to human health. Several flavonols are available to consumers as dietary supplements, promoted as anti-oxidants; however, incorporation of natural quercetin glycosides into food and beverage products has been limited by poor miscibility in water. Enzymatic conjugation of multiple glucose moieties to isoquercitrin to produce alpha-glycosyl isoquercitrin (AGIQ) enhances solubility and bioavailability. AGIQ is used in Japan as a food additive and has been granted generally recognized as safe (GRAS) status. However, although substantial genotoxicity data exist for quercetin, there is very little available data for AGIQ and isoquercitrin. To support expanded global marketing of food products containing AGIQ, comprehensive testing of genotoxic potential of AGIQ and isoquercitrin was conducted according to current regulatory test guidelines. Both chemicals tested positive in bacterial reverse mutation assays, and exposure to isoquercitrin resulted in chromosomal aberrations in CHO-WBL cells. All other in vitro mammalian micronucleus and chromosomal aberration assays, micronucleus and comet assays in male and female B6C3F1 mice and Sprague Dawley rats, and Muta™ Mouse mutation assays evaluating multiple potential target tissues, were negative for both chemicals. These results supplement existing toxicity data to further support the safe use of AGIQ in food and beverage products.

槲皮素及其糖苷对人类健康具有潜在的益处。几种黄酮醇可以作为膳食补充剂提供给消费者，并可以作为抗氧化剂推广。然而，天然槲皮素糖苷在食品和饮料产品中的掺入受到与水混溶性差的限制。多个葡萄糖部分与异槲皮苷的酶结合产生α-糖基异槲皮苷（AGIQ）可提高溶解度和生物利用度。AGIQ在日本用作食品添加剂，并已被普遍认为是安全（GRAS）状态。然而，尽管存在大量槲皮素的遗传毒性数据，但关于AGIQ和异槲皮苷的可用数据却很少。为了支持包含AGIQ的食品在全球范围内的扩展营销，根据当前的法规测试指南对AGIQ和异槲皮苷的遗传毒性潜力进行了全面测试。两种化学物质在细菌反向突变试验中均测试为阳性，并且暴露于异槲皮苷会导致CHO-WBL细胞中的染色体畸变。所有其他体外哺乳动物微核和染色体畸变测定，雄性和雌性B6C3F1小鼠和Sprague Dawley大鼠的微核和彗星测定以及评估多个潜在靶组织的Muta™小鼠突变测定对这两种化学物质均为阴性。这些结果补充了现有的毒性数据，以进一步支持AGIQ在食品和饮料产品中的安全使用。