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Immunomodulatory Activity of Docosahexenoic Acid on RAW264.7 Cells Activation through GPR120-Mediated Signaling Pathway
Journal of Agricultural and Food Chemistry ( IF 6.1 ) Pub Date : 2018-01-18 00:00:00 , DOI: 10.1021/acs.jafc.7b05894
Lirong Han 1 , Jun Yu 1 , Yuanyuan Chen 1 , Dai Cheng 1 , Xu Wang 1 , Chunling Wang 1
Affiliation  

In this study, we elucidated the immunomodulatory activity of docosahexaenoic acid (DHA) on protein expression in RAW264.7 cells and its molecular mechanism. The results showed that the proliferation index of RAW264.7 cells at 48 h was about 173.03 ± 7.82% after the treatment of 2.4 μM DHA. DHA could activate RAW264.7 cells by the G-protein coupled cell membrane receptor GPR120-C-Raf- mitogen-activated protein kinases (MAPKs)–nuclear factor κB (NF-κB) p65 pathway. In addition, 2.4 μM of DHA could significantly increase (P < 0.01) the mRNA and protein expression of inducible nitric oxide synthase (iNOS), which is consistent with the result of the NO release. ELISA results revealed that DHA could enhance the protein expression of cytokines IL-1β, IL-6, IL-10, IL-12, TNF-α, IFN-γ, and TGF-β. These results indicated that the immunomodulatory mechanism of RAW264.7 cells by DHA was associated with the release of NO and cytokines by stimulating the GPR120, C-Raf, and MAPKs to the NF-κB p65 pathway.

中文翻译:

二十二碳六烯酸对通过GPR120介导的信号通路对RAW264.7细胞活化的免疫调节活性

在这项研究中,我们阐明了二十二碳六烯酸(DHA)对RAW264.7细胞中蛋白质表达的免疫调节活性及其分子机制。结果表明,处理2.4μMDHA后,RAW264.7细胞在48 h的增殖指数约为173.03±7.82%。DHA可通过G蛋白偶联细胞膜受体GPR120-C-Raf-丝裂原活化蛋白激酶(MAPKs)-核因子κB(NF-κB)p65途径激活RAW264.7细胞。此外,2.4μM的DHA可能会显着增加(P<0.01)诱导型一氧化氮合酶(iNOS)的mRNA和蛋白表达,与NO释放的结果一致。ELISA结果表明,DHA可以增强细胞因子IL-1β,IL-6,IL-10,IL-12,TNF-α,IFN-γ和TGF-β的蛋白表达。这些结果表明,DHA通过刺激GPR120,C-Raf和MAPKs进入NF-κBp65途径,使DHA对RAW264.7细胞的免疫调节机制与NO和细胞因子的释放有关。
更新日期:2018-01-18
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