A cellulose degrading fungus Aspergillus fumigatus (CWSF-7) isolated from decomposed lignocellulosic waste containing soil was found to produce high titer of cellulases. The optimum activity of CMCase and FPase were 1.9 U/mL and 0.9 U/mL respectively while the highest protein concentration was found to be 1.2 mg/mL. Saccharification of two Pennisetum grass varieties [dennanath (DG) and hybrid napier grass (HNG)] were optimized using partially purified CMCase and FPase in equal concentration, i.e. a ratios of 1:1 and further with addition of commercial xylanase using response surface methodology (RSM). The production of total reducing sugar (TRS) using isolated cellulase were 396.6 and 355.8 (mg/g), whereas further addition of xylanase had higher TRS titers of 478.7 and 483.3 (mg/g) for DG and HNG respectively as evident from HPLC analysis. Further, characterization of the enzyme saccharified DG and HNG by SEM and ATR-FTIR revealed efficient hydrolysis of cellulose and partially hydrolysis of hemicellulose.

从含有土壤的分解木质纤维素废料中分离出的纤维素降解真菌烟曲霉（CWSF-7）被发现可产生高滴度的纤维素酶。CMCase和FPase的最佳活性分别为1.9 U / mL和0.9 U / mL，而最高蛋白质浓度为1.2 mg / mL。糖化两个狼尾草使用部分纯化的CMCase和FPase以相等的浓度（即1：1的比例）并进一步添加商业木聚糖酶（使用响应表面方法（RSM））优化了草木品种[dennanath（DG）和napier草（HNG）]。HPLC分析表明，使用分离的纤维素酶生产的总还原糖（TRS）分别为396.6和355.8（mg / g），而进一步添加木聚糖酶则DG和HNG的TRS滴度分别更高，分别为478.7和483.3（mg / g）。 。此外，通过SEM和ATR-FTIR表征糖化的DG和HNG的酶显示纤维素的有效水解和半纤维素的部分水解。