Cannabis misuse during pregnancy is associated with severe impacts on the mother and baby health, such as newborn low birth weight, growth restriction, pre-term birth, neurobehavioral and developmental deficits. In most of the cases, drug abuse is omitted or denied by the mothers. Thus, toxicological analyzes using maternal-fetal matrices takes place as a suitable tool to assess drug use. Herein, meconium was the chosen matrix to evaluate cannabis exposure through identification and quantification of 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic (THCCOOH). Accelerated solvent extraction (ASE) was applied for sample preparation technique to simultaneously extract and hydrolyze conjugated THCCOOH from meconium, followed by a solid-phase extraction (SPE) procedure. The method was developed and validated for gas chromatography–mass spectrometry (GC–MS), reaching hydrolysis efficiency of 98%. Limits of detection (LOD) and quantification (LOQ) were, respectively, 5 and 10 ng/g. The range of linearity was LOQ to 500 ng/g. Inter and intra-batch coefficients of variation were <8.4% for all concentration levels. Accuracy was in 101.7–108.9% range. Recovery was on average 60.3%. Carryover effect was not observed. The procedure was applied in six meconium samples from babies whose mothers were drug users and showed satisfactory performance to confirm fetal cannabis exposure.

大麻怀孕期间的滥用会严重影响母婴健康，例如新生儿体重不足，生长受限，早产，神经行为和发育缺陷。在大多数情况下，母亲会忽略或拒绝药物滥用。因此，使用母婴基质进行毒理学分析是评估药物使用情况的合适工具。在本文中，通过鉴定和定量11-nor-Δ9-四氢大麻酚-9-羧酸（THCCOOH），选择了胎粪作为评估大麻暴露的基质。加速溶剂萃取（ASE）用于样品制备技术，以从胎粪中同时萃取和水解共轭THCCOOH，然后进行固相萃取（SPE）程序。该方法已开发并通过了气相色谱-质谱（GC-MS）验证，达到98％的水解效率。检测限（LOD）和定量限（LOQ）分别为5 ng / g和10 ng / g。线性范围为LOQ至500 ng / g。对于所有浓度水平，批间和批内变异系数均<8.4％。准确性在101.7–108.9％的范围内。回收率平均为60.3％。未观察到残留效应。该程序应用于六个吸血胎粪样本，这些样本来自母亲为吸毒者的婴儿，并表现出令人满意的确认胎儿的性能。未观察到残留效应。该程序应用于六个吸血胎粪样本，这些样本来自母亲为吸毒者的婴儿，并表现出令人满意的确认胎儿的性能。未观察到残留效应。该程序应用于六个吸血胎粪样本，这些样本来自母亲为吸毒者的婴儿，并表现出令人满意的确认胎儿的性能。大麻暴露。