Vigabatrin is used as first line drug in treatment of infantile spasms for its potential benefit overweighing risk of causing permanent peripheral visual field defects and retinal damage. Chronic administration of vigabatrin in rats has demonstrated these ocular events are result of GABA accumulation and depletion of taurine levels in retinal tissues. In vigabatrin clinical studies taurine plasma level is considered as biomarker for studying structure and function of retina. The analytical method is essential to monitor taurine levels along with vigabatrin and GABA. A RP-HPLC method has been developed and validated for simultaneous estimation of vigabatrin, GABA and taurine using surrogate matrix. Analytes were extracted from human plasma, rat plasma, retina and brain by simple protein precipitation method and derivatized by naphthalene 2, 3‑dicarboxaldehyde to produce stable fluorescent active isoindole derivatives. The chromatographic analysis was performed on Zorbax Eclipse AAA column using gradient elution profile and eluent was monitored using fluorescence detector. A linear plot of calibration curve was observed in concentration range of 64.6 to 6458, 51.5 to 5150 and 62.5 to 6258 ng/mL for vigabatrin, GABA and taurine, respectively with r² ≥ 0.997 for all analytes. The method was successfully applied for estimating levels of vigabatrin and its modulator effect on GABA and taurine levels in rat plasma, brain and retinal tissue. This RP-HPLC method can be applied in clinical and preclinical studies to explore the effect of taurine deficiency and to investigate novel approaches for alleviating vigabatrin induced ocular toxicity.

Vigabatrin被用作治疗婴儿痉挛的一线药物，因为它的潜在益处超过了引起永久性周边视野缺损和视网膜损害的风险。长期向大鼠中施用vigabatrin证明这些眼部事件是GABA积累和视网膜组织中牛磺酸水平耗竭的结果。在vigabatrin的临床研究中，牛磺酸血浆水平被认为是研究视网膜结构和功能的生物标志物。该分析方法对于监测牛磺酸和vigabatrin和GABA的水平至关重要。已经开发了一种RP-HPLC方法，并验证了使用替代矩阵同时估算维加巴特林，GABA和牛磺酸的方法。通过简单的蛋白质沉淀法从人血浆，大鼠血浆，视网膜和大脑中提取分析物，并经萘2衍生化 3-二甲醛可以产生稳定的荧光活性异吲哚衍生物。使用梯度洗脱曲线在Zorbax Eclipse AAA色谱柱上进行色谱分析，并使用荧光检测器监控洗脱液。对于vigabatrin，GABA和牛磺酸，分别在64.6至6458、51.5至5150和62.5至6258 ng / mL的浓度范围内观察到校准曲线的线性图²  ≥0.997的所有分析物。该方法已成功用于估算维加巴特林的水平及其对大鼠血浆，脑和视网膜组织中GABA和牛磺酸水平的调节作用。该RP-HPLC方法可用于临床和临床前研究，以探索牛磺酸缺乏的影响并研究减轻vigabatrin引起的眼毒性的新方法。