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Optimized extraction of pectin-like polysaccharide from Suaeda fruticosa leaves: Characterization, antioxidant, anti-inflammatory and analgesic activities
Carbohydrate Polymers ( IF 11.2 ) Pub Date : 2018-01-06 , DOI: 10.1016/j.carbpol.2018.01.022
Zeineb Mzoughi , Amal Abdelhamid , Christophe Rihouey , Didier Le Cerf , Abderrahman Bouraoui , Hatem Majdoub

Box-Behnken design (BBD) was performed via ultrasound-assisted system to optimize extraction yield and total antioxidant ability of polysaccharides from Suaeda fruticosa. Three independent variables were studied: temperature, time and pH with optimal settings 90 °C, 37 min and 2.9, respectively. Under these settings, isolation yield and total antioxidant activity were 34.0% and 31.8 mg ascorbic acid equivalent/g material, respectively. Furthermore, preliminary structure study was carried out using UV–vis analysis, Fourier transform infrared spectroscopy (FT-IR) and Nuclear magnetic resonance (1D (1H, 13C NMR) and 2D (1H, 1H COSY, 1H/13C HSQC)) methods Size exclusion chromatography (SEC) determination revealed that polysaccharide extracted had an average molecular weight of 240 kDa. Chemical composition analyses showed that extracted polysaccharide was a pectin-like polysaccharide with uronic acid content (47.5%) and neutral monosaccharides (52.5%) constituted mainly of arabinose, mannose, galactose, rhamnose, glucose and xylose in the molar percentage of 48.63%, 5.85%, 29.42%, 7.96%, 6.54% and 1.60%, respectively. This sugar composition was typical of that of a galactoarabinan. Importantly, the optimum polysaccharide possesses significant potentials in antioxidant activity effect. Moreover, pharmacological investigation of extracted polysaccharide revealed a good anti-inflammatory effect and interesting peripheral as well as central antinociceptive activities.



中文翻译:

金盏菊叶片中优化提取果胶样多糖的特性,抗氧化,消炎和镇痛作用

通过超声辅助系统进行了Box-Behnken设计(BBD),以优化金盏花多糖的提取率和总抗氧化能力。研究了三个独立变量:最佳设置分别为90°C,37 min和2.9的温度,时间和pH。在这些设置下,分离收率和总抗氧化剂活性分别为34.0%和31.8 mg抗坏血酸当量/ g材料。此外,使用UV-vis分析,傅立叶变换红外光谱(FT-IR)和核磁共振(1D(1 H,13 C NMR)和2D(1 H,1 H COSY,1 H / 13体积排阻色谱法(SEC)测定表明,提取的多糖的平均分子量为240 kDa。化学成分分析表明,提取的多糖为果胶样多糖,其糖醛酸含量为47.5%,中性单糖为52.5%,主要成分是阿拉伯糖,甘露糖,半乳糖,鼠李糖,葡萄糖和木糖,摩尔百分比为48.63%,分别为5.85%,29.42%,7.96%,6.54%和1.60%。该糖成分是半乳糖阿拉伯聚糖的典型糖成分。重要的是,最佳多糖具有抗氧化活性作用的显着潜力。此外,提取多糖的药理研究显示出良好的抗炎作用以及有趣的外周以及中枢抗伤害感受活性。

更新日期:2018-01-06
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