In this study, the effects of UV-B radiation on the isoflavones accumulation, physiological and nutritional quality, water status, and characteristics of proteins in germinated soybeans were investigated. The results showed that isoflavones content in soybeans increased with appropriate intensity and time of UV-B radiation and decreased with excessive treatment. Fresh weight, length, free amino acids, reducing sugar contents and bulk water (T₂₃) in germinated soybeans decreased with increasing radiation time, indicating that UV-B inhibited the growth and nutrients metabolism of soybean during germination. Cell damage was detected in germinated soybeans with excessive UV-B radiation, as shown by the black spots in cotyledons and the increased intercellular water determined by LF-NMR. Germination resulted in an increase in random coil structures, while UV-B radiation induced no obvious changes in FT-IR spectrum and protein conformation of soybeans. Both UV-B radiation and germination caused the increase in soluble proteins, especially in 1.0–75.0 kDa fraction.

在这项研究中，研究了UV-B辐射对发芽大豆中异黄酮的积累，生理和营养质量，水分状况以及蛋白质特性的影响。结果表明，大豆中的异黄酮含量随适当的UV-B辐射强度和时间而增加，而随着过度处理而降低。新鲜的体重，长度，游离氨基酸，减少的糖含量和大量水分（T ₂₃随着辐射时间的增加，发芽大豆中的）降低，表明UV-B抑制了发芽过程中大豆的生长和养分代谢。如子叶上的黑点和通过LF-NMR测定的增加的细胞间水含量所显示，在过度UV-B辐射的发芽大豆中检测到细胞损伤。发芽导致无规卷曲结构的增加，而UV-B辐射并未引起FT-IR光谱和大豆蛋白质构象的明显变化。UV-B辐射和发芽都引起可溶性蛋白质的增加，尤其是1.0–75.0 kDa的分数。