An iTRAQ-based strategy was applied to investigate proteome changes in mud shrimp during long-term frozen storage under different conditions. A total of 226 proteins was identified as differential abundance proteins (DAPs) in mud shrimp from two frozen treatment groups (−20 °C and −40 °C) compared with the fresh control group. The proteome changes in mud shrimp muscle stored under −20 °C was much greater than that under −40 °C. Correlation analysis between DAPs and quality traits of mud shrimp muscle showed that 12 proteins were correlated closely with color (L^∗, a^∗, and b^∗ value) and texture (hardness, elasticity, and chewiness). Bioinformatic analysis revealed that most of these proteins were involved in protein structure, metabolic enzymes, and protein turnover. Among them, several proteins might be potential protein markers for color, and some proteins are good candidate predictors for textural properties of mud shrimp muscle.

基于iTRAQ的策略用于研究在不同条件下长期冷冻储存期间虾虾中蛋白质组的变化。与新鲜对照组相比，来自两个冷冻处理组（-20°C和-40°C）的泥虾中总共鉴定出226种蛋白质为差异丰度蛋白质（DAP）。在-20°C下储存的虾泥肌肉中的蛋白质组变化远大于在-40°C下储存的蛋白质组变化。DAPs与泥虾肌肉质量性状之间的相关性分析表明，12种蛋白质与颜色密切相关（L ^∗，a ^∗和b ^∗值）和质地（硬度，弹性和耐嚼性）。生物信息学分析表明，这些蛋白质大多数与蛋白质结构，代谢酶和蛋白质更新有关。其中，几种蛋白质可能是颜色的潜在蛋白质标记，而某些蛋白质是泥虾肌肉质地特性的良好候选预测指标。