Abstract Antimycotic behavior of zinc oxide decorated with silver nanoparticles (ZnO-AgNPs) against Trichophyton mentagrophytes (T. mentagrophytes) was investigated on this work. Silver decoration size was modulated during Ag2O and Ag0 synthesis in aqueous solution at pH 11, 12 and 14, obtaining the smallest size at pH 12 and the largest at pH 14. In ex-situ tests, ZnO-AgNPs prevents successfully the growth of T. mentagrophytes, the percent mycelial inhibition (PMI) of formula F12 was 91.76% while formulas F12 and F14 was 100%, they also not present toxicity in cell viability, even the formula F12 promoted cell growth. Additionally, once T. mentagrophytes has grown it turns in a hydrophobic biofilm, whose water contact angle (WCA) was 127 ± 3°, showing that no aqueous solution will have antimycotic activity against T. mentagrophytes biofilm. It has been propose a ZnO-AgNPs suspension in a hydrophobic fluid (polydimethylsiloxane, PDMS) that allowed to the particles permeate and permitted a direct contact with the biofilm, which was eliminated successfully. These results suggest that ZnO-AgNPs in a hydrophobic fluid emerge as a viable alternative for fungal infections treatment.

中文翻译：

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纳米银修饰氧化锌对须癣毛癣菌的抗真菌活性

摘要 这项工作研究了银纳米颗粒修饰的氧化锌 (ZnO-AgNPs) 对须癣毛癣菌 (T. mentagrophytes) 的抗真菌行为。在 pH 11、12 和 14 的水溶液中，Ag2O 和 Ag0 合成过程中调节银装饰尺寸，在 pH 12 时获得最小尺寸，在 pH 14 时获得最大尺寸。在非原位测试中，ZnO-AgNPs 成功阻止了 T 的生长对于须癣菌，配方F12的菌丝抑制百分比(PMI)为91.76%，而配方F12和F14为100%，它们在细胞活力方面也不存在毒性，甚至配方F12促进细胞生长。此外，一旦须癣菌生长，它就会变成疏水性生物膜，其水接触角 (WCA) 为 127 ± 3°，表明没有水溶液对须癣菌生物膜具有抗真菌活性。已经提出了在疏水流体（聚二甲基硅氧烷，PDMS）中的 ZnO-AgNPs 悬浮液，它允许颗粒渗透并允许与生物膜直接接触，这被成功消除。这些结果表明疏水流体中的 ZnO-AgNPs 成为治疗真菌感染的可行替代方案。