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Quantitation of trans-fatty acids in human blood via isotope dilution-gas chromatography-negative chemical ionization-mass spectrometry
Journal of Chromatography B ( IF 3 ) Pub Date : 2018-01-06 , DOI: 10.1016/j.jchromb.2017.12.038
Heather C. Kuiper , Na Wei , Samantha L. McGunigale , Hubert W. Vesper

Trans-fatty acids (TFA) are geometric isomers of naturally occurring cis-fatty acids. High dietary TFA intake has been associated with risk factors for cardiovascular disease. However, little is known about TFA levels in humans. To address this data need, we developed and validated a new isotope dilution-gas chromatography-negative chemical ionization-mass spectrometry (ID-GC-NCI-MS) method for quantitation of 27 fatty acids (FA) including 4 major TFA in human plasma, serum, and red blood cells (RBC) from 66 donors. Quantitation was performed with 18 isotope labeled internal standards and results are presented in μM and % of total FA. This method has high sensitivity and specificity due to use of pentafluorobenzyl-bromide derivatization combined with NCI-MS and a 200 m column to optimize positional and geometric FA isomer separation. The four major TFA, palmitelaidic acid, elaidic acid, trans-vaccenic acid, and linoelaidic acid, were detected in all samples, with median total TFA concentrations of 17.7 μM in plasma, 19.6 μM in serum, and 21.5 μM in RBC. The % of total FA for the TFA was 0.20% in plasma, 0.20% in serum, and 0.30% in RBC. Patterns for % FA are similar to those reported in other studies. We developed a highly specific, ID-GC-NCI-MS method to quantitate TFA and other FA in humans.



中文翻译:

同位素稀释-气相色谱-负化学电离-质谱法定量分析人血中反式脂肪酸

反式脂肪酸(TFA)是天然存在的顺式脂肪酸的几何异构体。饮食中高TFA摄入量与心血管疾病的危险因素有关。然而,人们对TFA水平的了解甚少。为了满足这一数据需求,我们开发并验证了一种新的同位素稀释-气相色谱-负化学电离质谱(ID-GC-NCI-MS)方法,用于定量测定人体血浆中27种脂肪酸(FA)包括4种主要的TFA ,来自66个捐献者的血清和红细胞(RBC)。用18种同位素标记的内标物进行定量,结果以μM和占总FA的百分比表示。该方法具有很高的灵敏度和特异性,这是由于将五氟苄基溴化物衍生化与NCI-MS结合使用,并使用200 m色谱柱来优化位置和几何FA异构体的分离。在所有样品中检测到四种主要的TFA,棕榈酸,依地酸,反式癸酸和亚油酸,血浆中的TFA总中位数浓度为17.7μM,血清中为19.6μM,RBC中为21.5μM。TFA的总FA含量在血浆中为0.20%,在血清中为0.20%,在RBC中为0.30%。FA的模式与其他研究报告的模式相似。我们开发了一种高度特异性的ID-GC-NCI-MS方法来定量人类中的TFA和其他FA。

更新日期:2018-01-06
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