AbstractThe authors describe an ultrasensitive method for simultaneous detection of neomycin (NEO) and quinolones antibiotics (QNS). It is based on the use of (a) two immuno-nanoprobes (a probe for NEO and a probe for QNS), (b) surface-enhanced Raman scattering (SERS) detection, and (c), a portable lateral flow assay (LFA). The two probes consist of gold nanoparticles (AuNPs) conjugated to the Raman active molecule 4-aminothiophenol (PATP), and to monoclonal antibody against NEO (NEO mAb) or against NOR (NOR mAb). Quantitative detection of NEO and QNS was realized via SERS of the PATP-coated AuNPs captured in the test line of a LFA. Under optimized condition, the visual limits of LFA are 10 ng·mL−1 for NEO and 200 ng·mL−1 for NOR, and with LODs down to 0.37 pg·mL−1 and 0.55 pg·mL−1 by using SERS. The NEO test line is not interfered by the NEO analogues gentamycin, streptomycin and tobramycin, but the NOR test line suffers from different degrees of cross-reactivity (CR) to 12 common other QNS, the CRs ranging from 1.5% to 136%. The recoveries of NEO and NOR from spiked milk samples ranged between 86% and 121%, with relative standard deviations (RSD) from 3% to 6%. The method is highly sensitive, accurate and effective. It may be applied to simultaneous detection of NEO and 8 QNS, including NOR, enoxacin, ciprofloxacin, ofloxacin, fleroxacin, marbofloxacin, enrofloxacin, and pefloxacin. Graphical abstractSchematic of a lateral flow assay (LFA) based on an indirect competitive model. By using two test lines, the LFA can detect the neomycin and quinolones antibiotics simultaneously. Based on the surface-enhanced Raman scattering (SERS), the LFA shows high sensitivity to antibiotics with low limit of detection.

中文翻译：

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一种基于 SERS 的多重免疫纳米探针，用于通过横向流动测定法超灵敏检测新霉素和喹诺酮类抗生素

摘要作者描述了一种同时检测新霉素 (NEO) 和喹诺酮类抗生素 (QNS) 的超灵敏方法。它基于使用 (a) 两个免疫纳米探针（一个用于 NEO 的探针和一个用于 QNS 的探针），(b) 表面增强拉曼散射 (SERS) 检测，以及 (c) 一种便携式侧向流分析（ LFA）。这两种探针由与拉曼活性分子 4-氨基苯硫酚 (PATP) 和抗 NEO (NEO mAb) 或抗 NOR (NOR mAb) 的单克隆抗体偶联的金纳米颗粒 (AuNP) 组成。NEO 和 QNS 的定量检测是通过 LFA 测试线中捕获的 PATP 涂层 AuNP 的 SERS 实现的。在优化条件下，LFA 的视觉极限对于 NEO 为 10 ng·mL-1，对于 NOR 为 200 ng·mL-1，并且通过使用 SERS，LOD 降至 0.37 pg·mL-1 和 0.55 pg·mL-1。NEO 测试线不受 NEO 类似物庆大霉素、链霉素和妥布霉素的干扰，但 NOR 测试线与 12 种常见的其他 QNS 存在不同程度的交叉反应（CR），CR 范围从 1.5% 到 136%。加标牛奶样品中 NEO 和 NOR 的回收率介于 86% 到 121% 之间，相对标准偏差 (RSD) 介于 3% 到 6% 之间。该方法灵敏、准确、有效。可用于同时检测NEO和8 QNS，包括NOR、依诺沙星、环丙沙星、氧氟沙星、氟罗沙星、马波沙星、恩诺沙星和培氟沙星。基于间接竞争模型的横向流动测定 (LFA) 的图形摘要示意图。通过使用两条检测线，LFA 可以同时检测新霉素和喹诺酮类抗生素。基于表面增强拉曼散射（SERS），