Hydrogen peroxide (H₂O₂), an important reactive oxygen species (ROS), is related to the oxidative stress in organisms, and plays important roles in a variety of cellular activities as well. So it is of crucial importance to develop sensitive and accurate sensing strategies to detect H₂O₂ in biological systems. Herein, by taking advantage of the unique emission characteristics of aggregation induced emission (AIE) fluorogens, we proposed a non-enzymatic fluorescence platform for facile and sensitive detection of H₂O₂, both in solution state using fluorescence spectrometer and on paper-based sensor via visual inspection. Through the reaction between L-cysteine and H₂O₂, the fluorescence of TPE-M-L, an AIE fluorogen formed between maleimide-functionalized tetraphenylethene (TPE-M) and L-cysteine, is quenched, and highly sensitive non-enzymatic H₂O₂ assay is readily carried out. The limit of detection (LOD) of 10 nM in solution state and 2.5 μM on paper-based sensor were obtained for H₂O₂ detection, which were superior or comparable to those previously reported in literature. Moreover, by integrating glucose oxidase with the AIE fluorogen of TPE-M-L, highly sensitive and selective glucose detection was also conveniently achieved both in solution state and on paper-based sensor by the as-proposed strategy, with the LODs of 50 nM in solution state and 10 μM via visual observation, much better than those obtained by other fluorescence methods. The as-proposed sensing strategy was also successfully applied to assay glucose in human serum samples. Therefore, the paper-based fluorescence sensor exhibits the advantages of simple fabrication, high sensitivity and portability, and has great potential to be applied in on-site assay of H₂O₂ and glucose in real samples.

过氧化氢（H ₂ O ₂）是一种重要的活性氧（ROS），与生物体内的氧化应激有关，并且在多种细胞活动中也起着重要的作用。因此，开发灵敏，准确的检测策略以检测生物系统中的H ₂ O ₂至关重要。本文中，通过利用聚集诱导发射（AIE）氟的独特发射特性，我们提出了一种非酶荧光平台，用于在溶液状态下使用荧光光谱仪和基于纸质的H ₂ O _2的灵敏检测。通过目测检查传感器。通过之间的反应淬灭L-半胱氨酸和H ₂ O ₂的荧光，即TPE-ML的荧光，TPE-ML是由马来酰亚胺官能化的四苯基乙烯（TPE-M）和L-半胱氨酸之间形成的AIE荧光，可进行高灵敏度的非酶H ₂ O ₂分析随手进行。对于H ₂ O _2，在溶液状态下的检测限（LOD）为10 nM，在纸基传感器上的检测限为2.5μM检测，优于或先前已报道过的文献。此外，通过将葡萄糖氧化酶与TPE-ML的AIE氟集成，还可以通过拟议的策略方便地在溶液状态和基于纸的传感器上实现高灵敏度和选择性的葡萄糖检测，溶液中的LOD为50 nM通过目测观察到10μM的状态，比其他荧光方法获得的结果要好得多。所提出的传感策略也已成功地应用于测定人血清样品中的葡萄糖。因此，该纸基荧光传感器具有制造简单，灵敏度高，便携性强的优点，在H ₂ O _2的现场检测中具有很大的潜力。 和真实样品中的葡萄糖。