Targeting the main three networking systems, viz. Las, RhI, and PQS, via natural quenchers is a new ray of hope for combating the persistent behavior of Pseudomonas aeruginosa. In the bacterial chemical vocabulary pyocyanin, N-AHLs and rhamnolipids are the main keywords, which are responsible for the social and nomadic behavior of P. aeruginosa. In the present work, LC-MS based real-time qualitative and quantitative analysis of pyocyanin, green phenazine, N-AHLs, and rhamnolipids were performed on P. aeruginosa PAO1. The quantitative analysis indicates that the production of pyocyanin and NHSLs increases with time while the production of rhamnolipids discontinued after 16 h. This indicates the emergence of persisters in the medium instead of planktonic cells. Rhamnolipids acting as a surfactant enhances the motility of the bacterial cells, whereas the pyocyanin is responsible for the biofilm formation. In a microtiter plate based assay, an effect of capsaicin and 6-gingerol was recorded. In the presence of capsaicin and 6-gingerol, a substantial decrease in the production of rhamnolipids, phenazine, quinolone, and N-AHLs was observed. Most interestingly, the 6-gingerol treatment led to a drastic decrease of rhamnolipids, phenazine, quinolone, and N-AHLs versus capsaicin. These studies demonstrate the effectiveness of the capsaicin and 6-gingerol on Las, PQS, and Rhl circuits in a bacterium in order to understand the persistent and social behavior. Here, we are reporting LC-MS/MS based qualitative and quantitative analysis of QS molecules by taking a low volume of culture (up to 200 μL). This method can be used as a platform to screen the new antivirulence agents for fighting the resistant behavior of P. aeruginosa during biofilm formation.

中文翻译：

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基于LCMS的群体感应抑制剂发现平台的建立：铜绿假单胞菌PAO1中的信号检测。

针对主要的三个网络系统，即。通过天然淬灭剂进行的Las，RhI和PQS为对抗铜绿假单胞菌的持续行为提供了新的希望。在细菌化学词汇绿脓素中，N-AHL和鼠李糖脂是主要关键词，它们负责铜绿假单胞菌的社会和游牧行为。在目前的工作中，对铜绿假单胞菌进行了基于LC-MS的实时定量和定量分析，测定了花青素，绿色吩嗪，N-AHL和鼠李糖脂。PAO1。定量分析表明，随着时间的推移，绿脓素和NHSLs的产量增加，而鼠李糖脂的产量在16小时后中断。这表明在培养基而不是浮游细胞中出现了持久性。鼠李糖脂作为表面活性剂可增强细菌细胞的运动能力，而花青素则负责生物膜的形成。在基于微量滴定板的测定中，记录了辣椒素和6-姜油醇的作用。在辣椒素和6-姜油醇的存在下，鼠李糖脂，吩嗪，喹诺酮和N-AHL的生成量显着降低。最有趣的是，与辣椒素相比，6-姜油治疗可显着降低鼠李糖脂，吩嗪，喹诺酮和N-AHLs的含量。这些研究证明了辣椒素和6-姜油醇对Las的有效性，PQS和Rhl在细菌中循环，以了解持久性和社交行为。在这里，我们报告了通过少量培养物（最多200μL）对QS分子进行LC-MS / MS的定性和定量分析。该方法可以作为筛选新的抗病毒剂对抗乙肝耐药性的平台。生物膜形成过程中的铜绿假单胞菌。